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由来生物
mouse
品質水準
結合体
unconjugated
抗体製品の状態
purified from hybridoma cell culture
抗体製品タイプ
primary antibodies
クローン
3B5H10, monoclonal
形状
buffered aqueous solution
化学種の反応性
human
包装
antibody small pack of 25 μL
濃度
~2 mg/mL
テクニック
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 1-2 μg/mL using extract of HEK-293T cells transfected with an N-terminal 171 amino acid fragment of human Huntingtin with a 68 glutamine stretch
アイソタイプ
IgG1
UniProtアクセッション番号
輸送温度
dry ice
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... HTT(3064)
詳細
Abnormal stretch of triplet CAG (or CAA) codon produces a homomeric repeat of glutamine residues beyond the critical threshold and will lead to neurodegenerative disease. Monoclonal mouse anti-polyglutamine antibody can be used to detect poly Q protein expression and in western blot. Monoclonal anti-polyglutamines antibody reacts specifically to homomeric polyglutamines independent of the species.
免疫原
GST-human Huntingtin (N-terminal fragment of 171 amino acids containing 65Q).
アプリケーション
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Monoclonal anti-polyglutamines antibody may be used for ELISA, immunoblot and slot blot. It can also be used in immunoprecipitation and immunocytochemistry.
物理的形状
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
法的情報
This product is for in vitro use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact the Regents of the University of California. This product is sold under license to U.S. Patent No. 6,291,652, owned by the Regents of the University of California.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
P1874-BULK:
P1874-100UL:
P1874-25UL:
P1874-VAR:
P1874-200UL:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Nicholas R Franich et al.
Journal of neuroscience research, 97(12), 1590-1605 (2019-07-10)
Huntington's disease (HD) is a progressive neurodegenerative disorder caused by an expanded CAG repeat within the huntingtin (HTT) gene. The Q140 and HdhQ150 knock-in HD mouse models were generated such that HdhQ150 mice have an expanded CAG repeat inserted into
Xuan Zhang et al.
Cellular and molecular neurobiology, 36(3), 459-470 (2016-03-10)
In Huntington's disease (HD) the imperfect expanded CAG repeat in the first exon of the HTT gene leads to the generation of a polyglutamine (polyQ) protein, which has some neuronal toxicity, potentially mollified by formation of aggregates. Accumulated research, reviewed
Veena Prahlad et al.
Proceedings of the National Academy of Sciences of the United States of America, 108(34), 14204-14209 (2011-08-17)
The consequence of chronic protein misfolding is the basis of many human diseases. To combat the deleterious effects of accumulated protein damage, all cells possess robust quality-control systems, specifically molecular chaperones and clearance machineries, that sense and respond to protein
Amy L Lee et al.
PloS one, 12(3), e0173644-e0173644 (2017-03-11)
Expanded polyglutamine repeats in different proteins are the known determinants of at least nine progressive neurodegenerative disorders whose symptoms include cognitive and motor impairment that worsen as patients age. One such disorder is Huntington's Disease (HD) that is caused by
Elizabeth Brooks et al.
Methods in molecular biology (Clifton, N.J.), 277, 103-128 (2004-06-18)
Expansion of a homomeric stretch of glutamine residues beyond a critical threshold can produce neurodegenerative disease. This observation led to the idea that abnormal polyglutamine stretches can alter protein structure in ways that contribute to disease. Because they are prone
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