P5994

ホスファターゼの基質

40 mg tablets

• 別名: 4-ニトロフェニルリン酸 二ナトリウム塩 六水和物, pNPP 二ナトリウム塩 六水和物
• 化学式: O₂NC₆H₄OP(O)(ONa)₂ · 6H₂O
• CAS番号: 333338-18-4
• 分子量: 371.14
• EC Number: 224-246-5
• MDL番号: MFCD00066288
• UNSPSCコード: 12352204
• PubChem Substance ID: 24898723
• NACRES: NA.54

特性

• 品質水準: 200
• フォーム: tablet
• 濃度: 36-44 mg/tablet
• 不純物: p-nitrophenol, essentially free
• 溶解性: H₂O: soluble, clear, colorless to yellow (1 tablet in 8 mL of water.)
• 保管温度: −20°C
• SMILES記法: O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1
• InChI: 1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2
• InChI Key: KAKKHKRHCKCAGH-UHFFFAOYSA-L

詳細

詳細

Human phosphatase-substrate networks are mapped based on the literature and protein-protein interaction (PPI) database. Based on gene expression data, the possible network of phosphatase-substrate-kinase is predicted.

アプリケーション

アルカリホスファタ-ゼおよび酸性ホスファタ-ゼによるELISA法で使用する呈色基質です。黄色い可溶性最終生成物を生じ、は405 nmで分光光度的に定量可能です。ｐNPP反応は、3N NaOHを用いて停止し、405 nmで吸光度を測定できます。

Phosphatase is a chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. It is been used for the colorimetric detection of CTB fusion proteins. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm.

Phosphatase substrate has been used as a substrate in alkaline phosphatase assay in serum samples, bone marrow stromal cells and human mesenchymal stem cells (hMSC).

The substrate has been used to determine the alkaline phosphatase (ALP) activity in human osteoblast-like Saos-2 cell lysates. ALP acts as an early marker of osteoblast differentiation that is expressed just before matrix mineralization begins.

数量

1錠あたり40 mgの基質を含有

調製ノート

Dissolve tablets to the desired concentration in either of the following buffers: 0.1 M glycine, pH 10.4, with 1 mM MgCl₂ and 1 mM ZnCl₂ or 1 M diethanolamine, pH 9.8, with 0.5 mM MgCl₂. Typically, a pNPP concentration of 1 mg/mL is used.

その他情報

酸性ホスファタ-ゼおよびアルカリホスファタ-ゼの定量用基質。

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, type N95 (US)