おすすめの製品
グレード
DNA grade
for molecular biology
品質水準
製品種目
BioReagent
フォーム
beads
膨張
1 g swells to 9-11 mL
ビーズ直径
50-150 μm
ポアサイズ
exclusion limit (20 bp dsDNA; (rA)20)
pH
2-10
その他の活性
DNase, RNase, none detected
保管温度
room temp
SMILES記法
O1C(C(C(C(C1CO)O)O)O)OCC2OC(C(C(C2O)O)O)OCC(O)C(O)C(O)C(O)C=O
InChI
1S/C18H32O16/c19-1-5(21)9(23)10(24)6(22)3-31-17-16(30)14(28)12(26)8(34-17)4-32-18-15(29)13(27)11(25)7(2-20)33-18/h1,5-18,20-30H,2-4H2
InChI Key
FZWBNHMXJMCXLU-UHFFFAOYSA-N
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詳細
Sephadex® G-50, Medium is an often-cited gel filtration medium for desalting and buffer exchange of biomolecules > 30,000 MW. Sephadex® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Medium” indicates a large particle size for use in large-scale group separations where high flow rates and low operating pressures are required.
アプリケーション
Sephadex® G-50 is suitable for spin column purification of DNA (from contaminating small molecules) for use in hybridizations. It was used to purify PCR products prior to DNA sequencing reactions.
Sephadex® G-50 has been used to purify the DNA products in cycle sequencing reactions.
特徴および利点
- Excellent recovery
- Minimal sample dilution
- Quick and easy to use
法的情報
Sephadex is a registered trademark of Cytiva
保管分類コード
11 - Combustible Solids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves, type N95 (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
S6022-25G:
S6022-VAR:
S6022-100G:
S6022-BULK:
Alisha K Holloway et al.
The American naturalist, 167(4), E88-101 (2006-05-04)
Polyploidization is one of the few mechanisms that can produce instantaneous speciation. Multiple origins of tetraploid lineages from the same two diploid progenitors are common, but here we report the first known instance of a single tetraploid species that originated
The history of a Nearctic colonization: molecular phylogenetics and biogeography of the Nearctic toads (Bufo)
Pauly GB, et al.
Evolution, 58(11), 2517-2535 (2004)
R Symula et al.
Molecular phylogenetics and evolution, 47(2), 569-580 (2008-03-20)
Geographic patterns of species diversity in southeast Australia have been attributed to changes in Pleistocene climate, but related phylogeographic patterns and processes are relatively understudied. 12S and 16S mitochondrial DNA sequences in Crinia signifera populations were used to infer historical
Mark Del Campo et al.
Journal of molecular biology, 389(4), 674-693 (2009-04-28)
The DEAD-box proteins CYT-19 in Neurospora crassa and Mss116p in Saccharomyces cerevisiae are broadly acting RNA chaperones that function in mitochondria to stimulate group I and group II intron splicing and to activate mRNA translation. Previous studies showed that the
Katharina Besemer et al.
Applied and environmental microbiology, 75(22), 7189-7195 (2009-09-22)
Streams are highly heterogeneous ecosystems, in terms of both geomorphology and hydrodynamics. While flow is recognized to shape the physical architecture of benthic biofilms, we do not yet understand what drives community assembly and biodiversity of benthic biofilms in the
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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