추천 제품
Quality Level
제품 라인
Novabiochem®
형태
solid
효능
>2000 mg/kg LD50, oral (Rat)
반응 적합성
reaction type: Biotinylations
제조업체/상표
Novabiochem®
pH
4.5 (0.1 g/L in H2O)
mp
232-233 °C
solubility
0.2 g/L
벌크 밀도
200‑500 kg/m3
응용 분야
peptide synthesis
저장 온도
2-8°C
InChI
1S/C10H16N2O3S/c13-8(14)4-2-1-3-7-9-6(5-16-7)11-10(15)12-9/h6-7,9H,1-5H2,(H,13,14)(H2,11,12,15)
InChI key
YBJHBAHKTGYVGT-UHFFFAOYSA-N
일반 설명
D(+)-Biotin is the simplest reagent for the introduction of biotin to peptides, proteins and other biomolecules. In SPPS, it is best coupled using DIPCDI/Oxyma Pure. Biotin-labeled peptides have many important applications in immunology and histochemistry, such as affinity purification and FRET-based flow cytometry, solid-phase immunoassays, and receptor localization, that exploit the high affinity of streptavidin and avidin for biotin.,,,
Associated Protocols and Technical Articles
Biotinylation Reagents for Peptide Synthesis
Literature references:
[1] K. Hofmann & Y. Kiso (1976) Proc. Natl. Acad. Sci., 73, 3516.
[2] T. Buranda, et al. (1999) Cytometry, 37, 21.
[3] I. Sélo, et al. (1996) J. Immunol. Methods, 199, 127.
[4] J. Howl, et al. (1993) Eur J Biochem., 213, 711.
Associated Protocols and Technical Articles
Biotinylation Reagents for Peptide Synthesis
Literature references:
[1] K. Hofmann & Y. Kiso (1976) Proc. Natl. Acad. Sci., 73, 3516.
[2] T. Buranda, et al. (1999) Cytometry, 37, 21.
[3] I. Sélo, et al. (1996) J. Immunol. Methods, 199, 127.
[4] J. Howl, et al. (1993) Eur J Biochem., 213, 711.
애플리케이션
Some recent applications of D(+)-Biotin in SPPS:
- In the high-throughput biotinylation of proteins. The approach for site-specific biotinylation of proteins is useful for various applications such as protein purification and analysis.
- In the site-specific biotinylation of multipodal glycoclusters on a solid support. The synthesis of biotinylated glycoclusters, has important applications in glycobiology.
법적 정보
Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
Peptides, antibodies, and FRET on beads in flow cytometry: A model system using fluoresceinated and biotinylated beta-endorphin
T. Buranda, et al.
Cytometry. Part A : the Journal of the International Society For Analytical Cytology, 37, 21-21 (1999)
Preferential labeling of alpha-amino N-terminal groups in peptides by biotin: application to the detection of specific anti-peptide antibodies by enzyme immunoassays
I. Selo, et al.
Journal of Immunological Methods, 199, 127-127 (1996)
Fluorescent and biotinylated linear peptides as selective bifunctional ligands for the V1a vasopressin receptor
J. Howl, et al.
European Journal of Biochemistry, 213, 711-711 (1993)
An approach to the targeted attachment of peptides and proteins to solid supports.
K. Hofmann & Y. Kiso
Proceedings of the National Academy of Sciences, 73, 3516-3516 (1976)
Synthesis of biotinylated multipodal glycoclusters on a solid support
Karskela M, et al.
European Journal of Organic Chemistry, 2012, 6594-6605 (2012)
문서
Biotin-labelled peptides find applications in immunology and histochemistry for affinity purification and receptor localization.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
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