AB3080

Anti-Green Fluorescent Protein Antibody

Chemicon^®, from rabbit

• 동의어(들): GFP, eGFP
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• 클론: polyclonal
• application: ELISA; ICC; IHC; WB
• 종 반응성: vertebrates
• 기술: ELISA: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• citations: 171

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 종 반응성: vertebrates
• 제조업체/상표: Chemicon^®
• 기술: ELISA: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• UniProt 수납 번호: P42212
• 배송 상태: dry ice
• 타겟 번역 후 변형: unmodified

설명

일반 설명

The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.

특이성

AB3080 is made against highly-purified native GFP from Aequorea victoria. It is reactive with GFP from both native and recombinant sources. While other commercial antibodies to GFP typically demonstrate strong reactivity with E. coli, the AB3080 GFP antibody has been purified by Protein A chromatography, followed by extensive adsorption against whole E. coli BL21 strain cells, giving them minimal cross-reactivity with E. coli.

면역원

Highly purified native GFP from Aequorea victoria.

애플리케이션

Research Category
Epitope Tags & General Use

Research Sub Category
Epitope Tags

This Anti-Green Fluorescent Protein Antibody is validated for use in ELISA, IC, IH, WB for the detection of Green Fluorescent Protein.

Western Blot:
A previous lot of this antibody was used in western blot.

ELISA:
A 1:2000-1:10,000 dilution of a previous lot was used in Enzyme-linked Immunosorbent Assay.

Note: While AB3080 has been adsorbed against E. coli proteins, most secondary antibodies have not. To reduce the likelihood of E. coli reactivity from a secondary antibody, we suggest the use of Millipore’s highly-purified secondary antibodies in indirect detection systems.

Immunocytochemistry:

Optimal working dilutions must be determined by end user.

품질

Routinely evaluated by immunocytochemistry on RENCX cells.

Immunocytochemistry Analysis: Confocal fluorescent analysis of RENCX constitutively expressed GFP using this lot of anti-GFP Rabbit pAB (Red). Green indicates the expressed GFP.

표적 설명

GFP is 27 kDa Dependent upon the molecular weight of the fusion protein being detected.

물리적 형태

Format: Purified

Protein A purified

Purified rabbit polyclonal IgG in 10 mM Tris buffer, pH 7.2 with 0.01% sodium azide.

저장 및 안정성

Stable for 1 year at -20ºC from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

분석 메모

Control
GFP fusion protein expressed in cells

RENCX cells

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable