P5511

Protein Kinase A from bovine heart

≥0.8 units/μg protein, lyophilized powder

• 동의어(들): Protein Kinase, 3′,5′-cyclic-AMP-dependent from bovine heart
• CAS Number: 9026-43-1
• 효소 위원회 번호: 2.7.1.37 (BRENDA, IUBMB)
• MDL number: MFCD00132121
• UNSPSC 코드: 12352204
• eCl@ss: 32160410
• NACRES: NA.77

속성

• 양식: lyophilized powder
• Quality Level: 200
• 특이 활성도: ≥0.8 units/μg protein
• 구성: Protein, ≥70%
• UniProt 수납 번호: P00517; P05131
• 저장 온도: −20°C
• 유전자 정보: cow ... PRKACA(282322) , PRKACB(282323)

설명

일반 설명

Protein Kinase A (PKA) is a serine/threonine kinase, which exists as a tetrameric holoenzyme.

애플리케이션

Protein Kinase A from bovine heart has been used to determine its activity using a molecular beacon probe. It has also been used to study the interaction of staurosporine with the ATP-binding site of kinases.

Protein Kinase A from bovine heart has been used to inhibit the phosphorylation of myosin.

생화학적/생리학적 작용

Many 3′,5′-cyclic AMP dependent protein kinases have been reported. Structural studies (Traugh, J.A., et al., Meth. Enzymol., 38, 290 [1974]) show the presence of at least two subunits, the regulatory subunit and the catalytic subunit. When both units are linked together, the catalytic activity is inhibited. However, when the cyclic-AMP binds to the regulatory subunit, the catalytic subunit is released and can then catalyze the transfer of phosphate from ATP to various proteins.

Protein Kinase A (PKA) inhibits hormone-sensitive lipase translocation from cytosol to storage droplets and blocks lipolysis. It regulates apoptosis, mitochondrial respiration and ATP synthesis. PKA is modulated by protease calpain.

포장

Package size based on protein content

단위 정의

One unit will transfer 1.0 picomole phosphate from ATP to hydrolyzed and partially dephosphorylated casein per minute at pH 6.5 at 30°C in the presence of cyclic AMP, determined by measuring the production of ADP.

물리적 형태

Crude lyophilized powder containing EDTA and potassium phosphate, pH 7.5.

제조 메모

Fractionated essentially by procedure of Gilman, A., Proc. Natl. Acad. Sci. USA, 67, 305 (1970).

분석 메모

Protein determined by biuret.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)