추천 제품
형태
lyophilized powder
Quality Level
특이 활성도
≥0.8 units/μg protein
구성
Protein, ≥70%
저장 온도
−20°C
유전자 정보
cow ... PRKACA(282322) , PRKACB(282323)
유사한 제품을 찾으십니까? 방문 제품 비교 안내
일반 설명
Protein Kinase A (PKA) is a serine/threonine kinase, which exists as a tetrameric holoenzyme.
애플리케이션
Protein Kinase A from bovine heart has been used to determine its activity using a molecular beacon probe. It has also been used to study the interaction of staurosporine with the ATP-binding site of kinases.
Protein Kinase A from bovine heart has been used to inhibit the phosphorylation of myosin.
생화학적/생리학적 작용
Protein Kinase A (PKA) inhibits hormone-sensitive lipase translocation from cytosol to storage droplets and blocks lipolysis. It regulates apoptosis, mitochondrial respiration and ATP synthesis. PKA is modulated by protease calpain.
Many 3′,5′-cyclic AMP dependent protein kinases have been reported. Structural studies (Traugh, J.A., et al., Meth. Enzymol., 38, 290 [1974]) show the presence of at least two subunits, the regulatory subunit and the catalytic subunit. When both units are linked together, the catalytic activity is inhibited. However, when the cyclic-AMP binds to the regulatory subunit, the catalytic subunit is released and can then catalyze the transfer of phosphate from ATP to various proteins.
포장
Package size based on protein content
단위 정의
One unit will transfer 1.0 picomole phosphate from ATP to hydrolyzed and partially dephosphorylated casein per minute at pH 6.5 at 30°C in the presence of cyclic AMP, determined by measuring the production of ADP.
물리적 형태
Crude lyophilized powder containing EDTA and potassium phosphate, pH 7.5.
제조 메모
Fractionated essentially by procedure of Gilman, A., Proc. Natl. Acad. Sci. USA, 67, 305 (1970).
분석 메모
Protein determined by biuret.
억제제
제품 번호
설명
가격
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
Simple fluorescence-based detection of protein kinase A activity using a molecular beacon probe
Journal of Bioenergetics and Biomembranes (2017)
Alkylation of Staurosporine to Derive a Kinase Probe for Fluorescence Applications
Dr. Alexandar
Chemistry of Materials (2016)
Cardiomyocyte Hypocontractility and Reduced Myofibril Density in End-Stage Pediatric Cardiomyopathy.
Ilse A E Bollen et al.
Frontiers in physiology, 8, 1103-1103 (2018-01-10)
Dilated cardiomyopathy amongst children (pediatric cardiomyopathy, pediatric CM) is associated with a high morbidity and mortality. Because little is known about the pathophysiology of pediatric CM, treatment is largely based on adult heart failure therapy. The reason for high morbidity
Signaling the signal: Protein Kinase a (PKA) inhibition by insulin-formed H2O2 and reactivation by thioredoxin
de Pina MZ, et al.
The Journal of Biological Chemistry (2008)
Nitroglycerine-and isoprenaline-induced vasodilatation: assessment from the actions of cyclic nucleotides
Itohy T, et al.
British Journal of Pharmacology, 84(2), 393-406 (1985)
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