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Merck
모든 사진(2)

주요 문서

SMB00610

Sigma-Aldrich

Lipopolysaccharide from Porphyromonas gingivalis

purified by phenol extraction

동의어(들):

LPS

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About This Item

UNSPSC 코드:
12352211
NACRES:
NA.25

생물학적 소스

Porphyromonas gingivalis

Quality Level

양식

powder

정제법

phenol extraction

불순물

≤3% Protein (Lowry)

색상

white to faint yellow

solubility

triethylene glycol dimethyl ether: 0.90-1.10 mg/mL to hazy, colorless to light yellow

배송 상태

ambient

저장 온도

2-8°C

일반 설명

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria like Porphyromonas gingivalis. It differs from LPS from Escherichia coli in its structure and various functional activities.

애플리케이션

Lipopolysaccharide has been used:

  • as a treatment to stimulate acute lung injury in human bronchial epithelial (HBE) cells
  • as an agonist for toll-like receptor 4 (TLR4) and to stimulate intestinal normal fibroblasts (NFs) to study osteopontin (OPN) expression in myofibroblasts
  • to study its effects on pro-inflammatory and pro-coagulant genes expression in endothelial cells

생화학적/생리학적 작용

LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular patterns (PAMPs).

Additionally, it was demonstrated that the mechanisms by which LPS from E. coli and P. gingivalis modulate cluster of differentiation 14 (CD14), toll-like receptor 2 (TLR2), and toll-like receptor 4 (TLR4) surface expression, primary and secondary cytokine responses are different.

Porphyromonas gingivalis is a Gram-negative bacterium that is known to be involved in adult periodontitis. Periodontitis is a chronic inflammatory disease characterized by the recession of the supportive tissue surrounding teeth. Studies have shown that the LPS from P. gingivalis plays an important role in this disease.
A recent study demonstrated that LPS from P. gingivalis stimulates insulin secretion by the pancreatic β cell line, MIN6. In the presence of 5 mM glucose and 50-500 ng/mL LPS from P. gingivalis, a significant induction of insulin secretion was observed.

제조 메모

LPS from P. gingivalis is soluble in water and cell culture medium DMEM (1-5 mg/mL), yielding a clear solution.

기타 정보

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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시험 성적서(COA)

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Epidemiology of subgingival bacterial pathogens in periodontal disease
Zambon, J. J., et al.
Molecular Pathogenesis of Periodontal Disease, 1, 1-12 (1994)
Diya Zhang et al.
Innate immunity, 14(2), 99-107 (2008-08-21)
Lipopolysaccharide (LPS) derived from the periodontal pathogen Porphyromonas gingivalis has been shown to differ from enterobacterial LPS in structure and function; therefore, the Toll-like receptors (TLRs) and the intracellular inflammatory signaling pathways are accordingly different. To elucidate the signal transduction
H E Barksby et al.
Clinical and experimental immunology, 156(3), 479-487 (2009-05-15)
Porphyromonas gingivalis lipopolysaccharide (LPS) (strain W50) interacts with Toll-like receptor 2 (TLR-2) leading to cytokine expression and inflammation, and thereby plays a key role in the pathogenesis of periodontal disease. The aims of this study were to investigate gene expression
Epidemiology of subgingival bacterial pathogens in periodontal disease.
Zambon, J.J., et al.
Molecular Pathogenesis of Periodontal Disease, 1-12 (1994)
Analysis of Lipopolysaccharides of Gram-Negative Bacteria
Mayer, H. et al.
Methods in Microbiology, 18, 157-207 (1985)

문서

Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.

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