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EZHS40

Millipore

High Sensitivity Human Amyloid β40 ELISA

This High Sensitivity Human Amyloid β40 ELISA is used to measure & quantify Amyloid β40 levels in Neuroscience research.

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Synonym(s):
Amyloid-beta protein 40
eCl@ss:
32161000

Quality Level

species reactivity

human

assay range

sensitivity: 6.0 pg/mL
(50 μl sample size)

standard curve range: 16-500 pg/mL

technique(s)

ELISA: suitable

detection method

colorimetric

Gene Information

human ... APP(351)

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EZHS42EZHSMABN11
Gene Information

human ... APP(351)

Gene Information

human ... APP(351)

Gene Information

human ... APP(351)

Gene Information

human ... APP(351)

assay range

sensitivity: 6.0 pg/mL
(50 μl sample size), standard curve range: 16-500 pg/mL

assay range

sensitivity: 8.0 pg/mL
(50 μl sample size), standard curve range: 16-500 pg/mL

assay range

-

assay range

-

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

-

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable, immunohistochemistry: suitable, western blot: suitable

General description

Amyloid beta peptides have been implicated in the etiology of Alzheimer’s disease. Amyloid beta 40 is the most prominent peptide and Amyloid beta 42 is the neurotoxic form. The Amyloid beta 42/40-ratio (AB ratio) has been reported as a better indicator of the Alzheimer pathology. Millipore’s High Sensitivity Human Amyloid β40 ELISA kit is used for the measurement of Amyloid β40 in cerebrospinal fluid, cell culture supernatants, primary neurons and plasma in a 96-well format.

Specificity

The Amyloid β40 ELISA (HS) uses monoclonal anti-Aβ antibodies with high selectivity for human Aβ. The capture antibody recognizes the C-terminal end of Amyloid β1-40, which causes a high selectivity for Aβ 40. The cross-reactivity of the used antibodies to other Amyloid peptides was tested by ELISA and BIACORE and shows no significant cross-reactivity to Aβ1-38, Aβ1-39, Aβ1-42, Aβ1-43 and Aβ1-44.

Application

Research Category
Neuroscience
Research Sub Category
Alzheimer′s Disease
This High Sensitivity Human Amyloid β40 ELISA is used to measure & quantify Amyloid β40 levels in Neuroscience research.
This assay requires 50 µl of sample (cerebrospinal fluid, cell culture supernatants, primary neurons, or plasma) and is an overnight assay.
Used to detect/quantify: Amyloid β40

Storage and Stability

Components in the kit can be stored up to 2 weeks at 2-8°C

Other Notes

Please contact Technical Service for linearity of dilution.

Disclaimer

For research use only. Not for use in diagnostic procedures.

pictograms

CorrosionExclamation mark

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Warning

Hazard Classifications

Aquatic Chronic 3 - Met. Corr. 1 - Skin Sens. 1

Storage Class

8A - Combustible, corrosive hazardous materials

wgk_germany

WGK 3


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Gunjan Manocha et al.
Current Alzheimer research, 15(12), 1123-1135 (2018-08-03)
Alzheimer's disease (AD) is associated with age-associated central nervous system degeneration and dementia. This decline in the function correlates with deposition of Aβ peptide containing plaques and associated reactive gliosis. The inflammatory phenotype of microglia, in particular, is often considered
Anna Bogstedt et al.
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Utilizing decision making biomarkers in drug development requires thorough assay validation. Special considerations need to be taken into account when monitoring biomarkers using immunoassays in the presence of therapeutic antibodies. We have developed robust and sensitive assays to assess target
Nataliya Golovyashkina et al.
Molecular neurodegeneration, 10, 60-60 (2015-11-07)
Dendritic simplification, a key feature of the neurodegenerative triad of Alzheimer's disease (AD) in addition to spine changes and neuron loss, occurs in a region-specific manner. However, it is unknown how changes in dendritic complexity are mediated and how they
Kendra L Puig et al.
Journal of Alzheimer's disease : JAD, 44(4), 1263-1278 (2014-11-20)
Alzheimer's disease (AD) is a neurodegenerative disorder histologically characterized by amyloid-β (Aβ) protein accumulation and activation of associated microglia. Although these features are well described in the central nervous system, the process and consequences of Aβ accumulation in the enteric
Angela M Floden et al.
Current Alzheimer research, 17(12), 1133-1144 (2021-01-20)
Beta amyloid (Aβ) peptide containing plaque aggregations in the brain are a hallmark of Alzheimer's Disease (AD). However, Aβ is produced by cell types outside of the brain suggesting that the peptide may serve a broad physiologic purpose. Based upon

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