51558

HEPES buffered saline

BioUltra, Molecular Biology, 2× concentrate

• Synonym(s): HEPES Saline Buffer Solution
• NACRES: NA.25
• PubChem Substance ID: 329757705
• UNSPSC Code: 12161700
• MDL number: MFCD00006158

Properties

• Product Name: HEPES buffered saline, BioUltra, Molecular Biology, 2× concentrate
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• SMILES string: OCCN1CCN(CC1)CCS(O)(=O)=O
• InChI key: JKMHFZQWWAIEOD-UHFFFAOYSA-N
• grade: Molecular Biology
• product line: BioUltra
• form: liquid
• quality: 2× concentrate
• composition: dextrose, 2.0 g/L ; HEPES, 10 g/L ; KCl, 0.74 g/L ; NaCl, 16 g/L ; Na₂HPO₄.2H₂O, 0.27 g/L
• concentration: 2 ×
• impurities: DNases, none detected; RNases, none detected; phosphatases, none detected; proteases, none detected
• refractive index: n20/D 1.339
• pH: 7.2 (25 °C)
• cation traces: Al: ≤1 mg/kg; Ba: ≤1 mg/kg; Bi: ≤1 mg/kg; Ca: ≤5 mg/kg; Cd: ≤1 mg/kg; Co: ≤1 mg/kg; Cr: ≤1 mg/kg; Cu: ≤1 mg/kg; Fe: ≤1 mg/kg; Li: ≤1 mg/kg; Mg: ≤1 mg/kg; Mn: ≤1 mg/kg; Mo: ≤1 mg/kg; Ni: ≤1 mg/kg; Pb: ≤1 mg/kg; Sr: ≤1 mg/kg; Zn: ≤1 mg/kg
• λ: neat
• UV absorption: λ: 260 nm A_max: 0.06; λ: 280 nm A_max: 0.04
• Quality Level: 200

Description

Application

HEPES buffered saline has been used:

• for the preparation of plasmid DNA
• as a reagent to prepare transfection master mix for DNA construct vectors
• to develop hydrogel precursor for cell leakage assay

General description

HEPES buffered saline is a tissue culture buffer, which is used to maintain the pH of cell culture. It is water soluble and atmosphere independent. HEPES destroys magnesium in sodium chloride solutions.

Preparation Note

concentration (in H₂O): 10 g/l HEPES; 16 g/l NaCl; 0.74 g/l KCl; 0.27 g/l Na₂HPO₄.2H₂O; 2.0 g/l dextrose

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves