51558
HEPES buffered saline
BioUltra, 2× concentrate
Synonym(s):
HEPES Saline Buffer Solution
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About This Item
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grade
Molecular Biology
Quality Level
product line
BioUltra
form
liquid
quality
2× concentrate
composition
dextrose, 2.0 g/L
HEPES, 10 g/L
KCl, 0.74 g/L
NaCl, 16 g/L
Na2HPO4.2H2O, 0.27 g/L
concentration
2 ×
impurities
DNases, none detected
RNases, none detected
phosphatases, none detected
proteases, none detected
refractive index
n20/D 1.339
pH
7.2 (25 °C)
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General description
HEPES buffered saline is a tissue culture buffer, which is used to maintain the pH of cell culture. It is water soluble and atmosphere independent. HEPES destroys magnesium in sodium chloride solutions.
Application
HEPES buffered saline has been used:
- for the preparation of plasmid DNA
- as a reagent to prepare transfection master mix for DNA construct vectors
- to develop hydrogel precursor for cell leakage assay
Preparation Note
concentration (in H2O): 10 g/l HEPES; 16 g/l NaCl; 0.74 g/l KCl; 0.27 g/l Na2HPO4.2H2O; 2.0 g/l dextrose
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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