Skip to Content
MilliporeSigma
All Photos(1)

Documents

51309

Sigma-Aldrich

TRIS borate-EDTA buffer concentrate, long run

for Northern and Southern blotting, solution

Sign Into View Organizational & Contract Pricing

MDL number:
UNSPSC Code:
12161700
NACRES:
NA.25

grade

for molecular biology

Quality Level

product line

BioReagent

form

solution

quality

10× concentrate
ready-to-use solution

impurities

DNases, none detected
RNases, none detected
phosphatases, none detected
proteases, none detected

storage temp.

room temp

Related Categories

General description

TBE (Tris/Borate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution is effective under slightly basic conditions, which keeps DNA deprotonated , water-soluble, and protected from degradation. This concentrate can be easily diluted to 1x or 0.5x before use (with molecular biology grade water).

Application

Suitable for use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures.

Components

The TBE 10x concentrate contains 1.3M TRIS, 450 mM boric acid and 25 mM EDTA in water.

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Repr. 1B

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service