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GE17-0404-01

HiTrap® Protein G High Performance

Cytiva 17-0404-01, pack of 5 × 1 mL

Synonym(s):

Protein purification column

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About This Item

UNSPSC Code:
23151816
NACRES:
NA.56

ligand

recombinant protein G lacking albumin-binding region

form

prepacked column

crosslinking

cross-linked

packaging

pack of 5 × 1 mL

manufacturer/tradename

Cytiva 17-0404-01

storage condition

(20% Ehtanol)
 (20% Ethanol)

parameter

<4 mL/min flow rate
42 psi

technique(s)

liquid chromatography (LC): suitable

bed size

7 mm × 25 mm

bed volume

1 mL

column I.D.

7 mm

size

matrix

(cross-linked agarose, spherical)

matrix active group

Protein G functional group

avg. part. size

34 μm

cleaning

2-9

working range

3-9

capacity

>1 mg binding capacity (lgG/column)
 25 mg binding capacity(human IgG/column)

separation technique

affinity

storage temp.

2-8°C

General description

HiTrap® Protein G HP columns are prepacked with Protein G Sepharose™ High Performance for fast, easy separations of IgG from different species and subclasses.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Features and Benefits

  • High binding capacity of 25 mg IgG/mL medium and fast binding kinetics.
  • 34 μm bead size for high performance, high-resolution purifications.
  • Binds broad range of different species and subclasses of IgG.
  • Simple and proven method giving reproducible results.
  • Designed for use with syringe, peristalic pump, or chromatography system.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

related product

Product No.
Description
Pricing

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Storage Class

3 - Flammable liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Lin-Ping Wu et al.
Nature communications, 12(1), 4858-4858 (2021-08-13)
Complement is an enzymatic humoral pattern-recognition defence system of the body. Non-specific deposition of blood biomolecules on nanomedicines triggers complement activation through the alternative pathway, but complement-triggering mechanisms of nanomaterials with dimensions comparable to or smaller than many globular blood

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This page shows how to prepare samples for purification with affinity chromatography.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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