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MAK174

Sigma-Aldrich

Fatty Acid Extraction Kit, Low Standard

greener alternative

sufficient for 40 extractions

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NACRES:
NA.25

usage

sufficient for 40 extractions

greener alternative product characteristics

Waste Prevention
Learn more about the Principles of Green Chemistry.

application(s)

cosmetics
food and beverages
sample preservation

greener alternative category

relevant disease(s)

cancer

storage temp.

room temp

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room temp

storage temp.

room temp

storage temp.

−20°C

storage temp.

room temp

greener alternative product characteristics

Waste Prevention
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

Waste Prevention
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

-

greener alternative product characteristics

-

application(s)

cosmetics
food and beverages
sample preservation

application(s)

cosmetics
food and beverages
sample preservation

application(s)

-

application(s)

cosmetics
food and beverages
sample preservation

greener alternative category

, Aligned

greener alternative category

Aligned,

greener alternative category

-

greener alternative category

-

relevant disease(s)

cancer

relevant disease(s)

-

relevant disease(s)

endocrinological disorders, diabetes; gastrointestinal diseases; cancer

relevant disease(s)

-

General description

Lipids are structural components of cell membranes that play a critical role in gene transcription, signaling, and metabolism. Several lipid species exist in biological systems, including phospholipids, triglycerides, and fatty acids. When extracting fatty acids at low or very low levels, such as in cellular matrices, use the MAK174 fatty acid extraction kit. When extracting fatty acids at normal or high levels, such as in food matrices, use the MAK338 fatty acid extraction kit.
The Folch method has been conventionally used to extract lipids containing fatty acids from biological samples, using chloroform, methanol, and water to separate lipids from aqueous-soluble compounds. In this procedure, lipids are retained in the lower chloroform layer; whereas, aqueous-soluble compounds are retained in the upper methanol-water layer. The sample is then centrifuged to achieve uniform separation and the bottom chloroform layer is transferred with a pipette to a new test tube.

Application

Fatty Acid Extraction Kit has been used for fatty acid extraction.

Suitability

This kit is suitable for the extraction of lipids from a variety of samples such as whole blood, serum, biological tissue, food samples, and oil.

Principle

The Fatty Acid Extraction Kit shortens this extraction process by eliminating the need to prepare solvents and standards, centrifugation, and pipetting. Once the sample is homogenized and dissolved in the Extraction Solvent supplied in our kit, it is inverted twice and poured into the syringe containing a filter, which preferentially elutes the chloroform layer containing total lipids. A portion of the total lipid extract containing fatty acids can then be transesterified for GC-FID analysis.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product reduces solvent waste over traditional methods. Find details here.

Other Notes

Experts have tested the Fatty Acid Extraction Kit. Watch the Video to learn more and to see how the technology works.

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Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Flam. Liq. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 - STOT SE 1 - STOT SE 3

target_organs

Central nervous system, Eyes

Storage Class

3 - Flammable liquids

wgk_germany

WGK 3

flash_point_f

49.5 °F - closed cup

flash_point_c

9.7 °C - closed cup


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Zhichao Zhang et al.
Journal of food science, 82(10), 2291-2297 (2017-08-24)
Fatty acid extraction methods are time-consuming and expensive because they involve multiple steps and copious amounts of extraction solvents. In an effort to streamline the fatty acid extraction process, this study compared the standard Folch lipid extraction method to a
Liquid Extraction: Folch
Markus R W
Encyclopedia of Lipidomics (2016)
Structure of the Plasma Membrane
Cooper G M.
The Cell: A Molecular Approach (2000)
Laura Inés Elvira-Torales et al.
International journal of molecular sciences, 20(7) (2019-04-17)
The purpose of this work was to evaluate the effect of dietary carotenoids from spinach on the inflammation and oxidative stress biomarkers, liver lipid profile, and liver transcriptomic and metabolomics profiles in Sprague-Dawley rats with steatosis induced by a high-fat
Laura Inés Elvira-Torales et al.
Antioxidants (Basel, Switzerland), 9(11) (2020-10-30)
Non-alcoholic fatty liver disease (NAFLD) is currently a serious and growing clinical problem in developed and developing countries and is considered one of the most frequent chronic liver diseases in the world. The aim of this study was to evaluate

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