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MAK338

Sigma-Aldrich

Fatty Acid Extraction Kit, High Standard

greener alternative

sufficient for 40 extractions

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usage

sufficient for 40 extractions

greener alternative product characteristics

Waste Prevention
Learn more about the Principles of Green Chemistry.

application(s)

cosmetics
food and beverages
sample preparation

greener alternative category

relevant disease(s)

cancer

storage temp.

room temp

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This Item
MAK224MAK156MAK044
vibrant-m

MAK224

Fatty Acid Methylation Kit

vibrant-m

MAK156

Fatty Acid Uptake Kit

vibrant-m

MAK044

Free Fatty Acid Quantitation Kit

storage temp.

room temp

storage temp.

room temp

storage temp.

−20°C

storage temp.

−20°C

greener alternative product characteristics

Waste Prevention
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

-

greener alternative product characteristics

-

greener alternative product characteristics

-

application(s)

cosmetics
food and beverages
sample preparation

application(s)

cosmetics
food and beverages
sample preservation

application(s)

-

application(s)

cosmetics
food and beverages

greener alternative category

Aligned,

greener alternative category

-

greener alternative category

-

greener alternative category

-

relevant disease(s)

cancer

relevant disease(s)

-

relevant disease(s)

endocrinological disorders, diabetes; gastrointestinal diseases; cancer

relevant disease(s)

endocrinological disorders, diabetes; cancer

General description

Lipids are structural components of cell membranes that play a critical role in gene transcription, signaling, and metabolism. Several lipid species exist in biological systems, including phospholipids, triglycerides, and fatty acids.

When extracting fatty acids at normal or high levels, such as in food matrices, use the MAK338 Fatty Acid Extraction Kit. This kit produces stronger signals. When extracting fatty acids at low or very low levels, such as in cellular matrices, use the MAK174 Fatty Acid Extraction Kit. If in doubt about which kit to use, use MAK338. The Folch method has been conventionally used to extract lipids containing fatty acids from biological samples, using chloroform, methanol, and water to separate lipids from aqueous-soluble compounds. In this procedure, lipids are retained in the lower chloroform layer; whereas, aqueous-soluble compounds are retained in the upper methanol-water layer. The sample is then centrifuged to achieve uniform separation and the bottom chloroform layer is transferred with a pipette to a new test tube.

The Fatty Acid Extraction Kit shortens this extraction process by eliminating the need to prepare solvents and standards, centrifugation, and pipetting. Once the sample is homogenized and dissolved in the Extraction Solvent supplied in the kit, it is inverted twice and poured into the syringe containing a filter, which preferentially elutes the chloroform layer containing total lipids. A portion of the total lipid extract containing fatty acids can then be transesterified for GC-FID analysis.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product reduces solvent waste over traditional methods. Find details here.

Suitability

This kit is suitable for the extraction of lipids from a variety of samples such as whole blood, serum, biological tissue, food samples, and oil.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Related product

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Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Flam. Liq. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 - STOT SE 1 - STOT SE 3

target_organs

Central nervous system, Eyes

Storage Class

3 - Flammable liquids

wgk_germany

WGK 3

flash_point_f

95.0 °F

flash_point_c

35 °C


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