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RAB0202

Sigma-Aldrich

Glucagon EIA Kit

for serum, plasma, culture supernatant and cell lysates

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NACRES:
NA.32

species reactivity

human, rat, mouse

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
enzyme immunoassay: suitable

input

sample type serum
sample type cell lysate
sample type culture supernatant(s)
sample type plasma

assay range

inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 4.77 pg/mL
standard curve range: 1–1000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... GCG(2641)

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packaging

kit of 96 wells (12 strips x 8 wells)

packaging

kit of 96 wells (12 strips x 8 wells)

packaging

kit of 96 wells (12 strips x 8 wells)

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable, enzyme immunoassay: suitable

technique(s)

ELISA: suitable, enzyme immunoassay: suitable

technique(s)

ELISA: suitable, enzyme immunoassay: suitable

technique(s)

ELISA: suitable, enzyme immunoassay: suitable

input

sample type serum
sample type cell lysate
sample type culture supernatant(s)
sample type plasma

input

sample type culture supernatant(s)
sample type cell lysate
sample type plasma
sample type serum

input

sample type serum
sample type cell lysate
sample type plasma
sample type culture supernatant(s)

input

sample type serum
sample type culture supernatant(s)
sample type plasma
sample type cell lysate

assay range

inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 4.77 pg/mL
standard curve range: 1–1000 pg/mL

assay range

inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 0.1 pg/mL
standard curve range: 0.1–1000 pg/mL

assay range

inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 1.02 pg/mL
standard curve range: 0.1-1000 pg/mL

assay range

inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 1.66 pg/mL
standard curve range: 0.1–1000 pg/mL

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

General description

The Glucagon Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting Glucagon peptide based on the principle of Competitive Enzyme Immunoassay.

Immunogen

Glucagon , synthetic peptide

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Glucagon EIA Kit has been used in the quantification of glucagon in mice serum. It has also been used to quantitate glucagon in rat plasma.

Preparation Note

The recommended dilution for normal Serum and Plasma is 2 fold for this product.

Kit Components Also Available Separately

Product No.
Description
SDS

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3


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Firdevs Mor et al.
Toxins, 9(4) (2017-04-20)
In this study, the diabetogenic effects of long term Ochratoxin A (OTA) administration in rats were investigated, and its role in the etiology of diabetes mellitus (DM) was examined utilizing 42 female Wistar rats for these purposes. The rats were
High mobility group protein B1 controls liver cancer initiation through yes-associated protein-dependent aerobic glycolysis
Chen R, et al.
Hepatology, 67(5), 1823-1841 (2018)
Deletion of astrocytic BMAL1 results in metabolic imbalance and shorter lifespan in mice
Barca-Mayo O, et al.
Glia, 68(6), 1131-1147 (2020)
Rachel J Perry et al.
The Journal of clinical investigation, 127(2), 657-669 (2017-01-24)
The mechanism by which leptin reverses diabetic ketoacidosis (DKA) is unknown. We examined the acute insulin-independent effects of leptin replacement therapy in a streptozotocin-induced rat model of DKA. Leptin infusion reduced rates of lipolysis, hepatic glucose production (HGP), and hepatic
Diabetogenic Effects of Ochratoxin A in Female Rats
Mor F, et al.
Toxins, 9(4), 144-144 (2017)

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