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RAB0671

Sigma-Aldrich

Human GCG / Glucagon ELISA Kit

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NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable

input

sample type plasma
sample type serum
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 2.5 pg/mL
standard curve range: 2.03-130 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... GCG(2641)

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Sigma-Aldrich

RAB0671

Human GCG / Glucagon ELISA Kit

Human GCP-2 / CXCL6&#160; ELISA Kit for serum, plasma, cell culture supernatant and urine

RAB0134

Human GCP-2 / CXCL6  ELISA Kit

Gene Information

human ... GCG(2641)

Gene Information

human ... GCG(2641)

Gene Information

human ... GCG (1-37)(2641)

Gene Information

human ... CXCL6(6372)

input

sample type plasma
sample type serum
sample type cell culture supernatant(s)

input

-

input

sample type serum
sample type plasma
sample type cell culture supernatant(s)

input

sample type urine
sample type cell culture supernatant(s)
sample type plasma
sample type serum

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 2.5 pg/mL
standard curve range: 2.03-130 pg/mL

assay range

-

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 2.3 pg/mL
standard curve range: 2.34-150 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 45 pg/mL
standard curve range: 39.06-2500 pg/mL

detection method

colorimetric

detection method

-

detection method

colorimetric

detection method

colorimetric

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

General description

Glucagon-like peptide-1 (GLP-1), a peptide hormone is liberated from endocrine epithelial cells of the small intestine. The proglucagon gene (GCG) codes for glucagon, glucagon-like peptide-1 (GLP-1), and glucagon-like peptide-2 (GLP-2).

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Glucagon-like peptide-1 (GLP-1) treatment helps to lower the risk of stroke in diabetic patients and to be neuroprotective in experimental stroke models. It participates in glucose homeostasis and has an impact on the cardiovascular system. GLP-1 may promote pancreatic β-cell proliferation and neogenesis. It enhances insulin release after a meal and triggers insulin transcription and biosynthesis.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

Storage Class

8A - Combustible, corrosive hazardous materials

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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25G
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Tianru Jin
The Journal of endocrinology, 198(1), 17-28 (2008-06-26)
The proglucagon gene (gcg) encodes a number of peptide hormones that are of cell-type specifically expressed in the pancreatic islets, the distal ileum and the large intestine, as well as certain brain neuronal cells. These hormones are important in controlling
Charles-Étienne Plourde et al.
Obesity (Silver Spring, Md.), 22(8), 1838-1846 (2014-04-25)
To assess the rapid improvement of insulin sensitivity and β-cell function following biliopancreatic diversion with duodenal switch (BPD-DS) and determine the role played by caloric restriction in these changes. Standard meals were administrated before and on day 3, 4, and
David R Powell et al.
The Journal of pharmacology and experimental therapeutics, 350(2), 232-242 (2014-05-23)
Treatments that lower blood glucose levels and body weight should benefit patients with type 2 diabetes mellitus (T2DM). We developed LX4211 [(2S,3R,4R,5S,6R)-2-(4-chloro-3-(4-ethoxybenzyl)phenyl)-6-(methylthio)tetrahydro-2H-pyran-3,4,5-triol], an orally available small molecule that decreases postprandial glucose excursions by inhibiting intestinal sodium/glucose cotransporter 1 (SGLT1) and
Cristina Barale et al.
Thrombosis research, 180, 74-85 (2019-06-24)
The incretin hormone Glucagon-like peptide 1(GLP-1) plays a pivotal role in maintaining glucose homeostasis with effects also on the cardiovascular system. GLP-1 influences platelet functions by increasing the inhibitory action of nitric oxide (NO) and reducing oxidative stress. To date
Martin Larsson et al.
Cardiovascular diabetology, 18(1), 91-91 (2019-07-17)
Glucagon-like peptide-1 (GLP-1) treatment has been shown to reduce stroke incidence in diabetes and also to be neuroprotective in experimental stroke models. The prognostic value of endogenous levels of GLP-1 in the recovery phase after stroke remains to be elucidated. The

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