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T3069

Sigma-Aldrich

Trizma® hydrochloride solution

pH 8.0, BioPerformance Certified, 2 M, suitable for cell culture

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Synonym(s):
Tris hydrochloride solution
NACRES:
NA.25

grade

BioPerformance Certified
for molecular biology

Quality Level

form

solution

concentration

2 M

technique(s)

cell culture | mammalian: suitable

impurities

DNase, RNase, Protease, none detected
bioburden, tested
endotoxin, tested
≤5 ppm Heavy metals (as Pb)

pH

8.0

useful pH range

7.0-9.0

absorption

≤0.05 at 290 at 40%

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This Item
T2569T2444T2819
vibrant-m

T3069

Trizma® hydrochloride solution

Premium Grade
vibrant-m

T2569

Trizma® hydrochloride solution

Premium Grade
vibrant-m

T2444

Trizma® hydrochloride solution

Premium Grade
vibrant-m

T2819

Trizma® hydrochloride solution

Premium Grade
grade

BioPerformance Certified, for molecular biology

grade

BioPerformance Certified, for molecular biology

grade

BioPerformance Certified, for molecular biology

grade

BioPerformance Certified, for molecular biology

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

concentration

2 M

concentration

1 M

concentration

1 M

concentration

1 M

form

solution

form

solution

form

solution

form

solution

pH

8.0

pH

7.8

pH

7.6

pH

9.0

General description

Tris(hydroxymethyl)aminomethane (Tris) has a pH buffering range between 7.0 - 9.0 and is a routine buffer in biological sciences. Tris is used majorly in combination with ethylenediaminetetraacetic acid (EDTA), especially for electrophoretic studies. It displays negligible binding towards metal in solutions.
A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units.

Application

Trizma® hydrochloride solution has been used as a component of lysis buffer for testing on commercially available biological sample preservation paper types. It has also been used as a vehicle and for dissolving leptin in reporter gene assay studies.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

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25G
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705578-5MG-PW

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MMYOMAG-74K-13

1000309185

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