MilliporeSigma
  • [Blood plasma protein adsorption capacity of perfluorocarbon emulsion stabilized by proxanol 268 (in vitro and in vivo studies)].

[Blood plasma protein adsorption capacity of perfluorocarbon emulsion stabilized by proxanol 268 (in vitro and in vivo studies)].

Biofizika (2012-05-19)
A N Sklifas, V K Zhalimov, A A Temnov, N I Kukushkin
ABSTRACT

The adsorption abilities of the perfluorocarbon emulsion stabilized by Proxanol 268 were investigated in vitro and in vivo. In vitro, the saturation point for the blood plasma proteins was nearly reached after five minutes of incubation of the emulsion with human/rabbit blood plasma and was stable for all incubation periods studied. The decrease in volume ratio (emulsion/plasma) was accompanied by the increase in the adsorptive capacity of the emulsion with maximal values at 1/10 (3.2 and 1.5 mg of proteins per 1 ml of the emulsion, for human and rabbit blood plasma, respectively) that was unchanged at lower ratios. In vivo, in rabbits, intravenously injected with the emulsion, the proteins with molecular masses of 12, 25, 32, 44, 55, 70, and 200 kDa were adsorbed by the emulsion (as in vitro) if it was used 6 hours or less before testing. More delayed testing (6 h) revealed elimination of proteins with molecular masses of 25 and 44 kDa and an additional pool of adsorpted new ones of 27, 50, and 150 kDa. Specific adsorptive capacity of the emulsion enhanced gradually after emulsion injection and reached its maximum (3.5-5 mg of proteins per 1 ml of the emulsion) after 24 hours.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol), average Mn ~2,000
Sigma-Aldrich
Poloxamer 188 solution, 10%, sterile-filtered, BioReagent, suitable for insect cell culture
Sigma-Aldrich
Kolliphor® P 407, oxyethylene 71.5-74.9 %
Sigma-Aldrich
Kolliphor® P 407 micro
Sigma-Aldrich
Kolliphor® P 188 micro
Sigma-Aldrich
Kolliphor® P 188, solid
Sigma-Aldrich
Synperonic® PE/P84
Sigma-Aldrich
Poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol), average Mn ~14,600
Sigma-Aldrich
Poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol), average Mn ~4,400
Sigma-Aldrich
Poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol), average Mn ~2,900
Sigma-Aldrich
Poly(propylene glycol)-block-poly(ethylene glycol)-block-poly(propylene glycol), average Mn ~2,000
Sigma-Aldrich
Poly(propylene glycol)-block-poly(ethylene glycol)-block-poly(propylene glycol), average Mn ~3,300
Sigma-Aldrich
Poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol), average Mn ~5,800
Sigma-Aldrich
Poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol), average Mn ~8,400
Sigma-Aldrich
Poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol), average Mn ~1,900
Sigma-Aldrich
Poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol), average Mn ~1,100
Sigma-Aldrich
Synperonic® PE P105, surfactant
Sigma-Aldrich
Kolliphor® P 188
Sigma-Aldrich
Poloxamer 407, purified, non-ionic
Sigma-Aldrich
Synperonic® F 108, surfactant, non-ionic
Sigma-Aldrich
Pluronic® F-127, powder, BioReagent, suitable for cell culture