Skip to Content
MilliporeSigma
  • Correlations of amyloid-β concentrations between CSF and plasma in acute Alzheimer mouse model.

Correlations of amyloid-β concentrations between CSF and plasma in acute Alzheimer mouse model.

Scientific reports (2014-10-28)
Soo Min Cho, Hyunjin Vincent Kim, Sejin Lee, Hye Yun Kim, Woong Kim, Tae Song Kim, Dong Jin Kim, YoungSoo Kim
ABSTRACT

Amyloid-β (Aβ) is one of the few neuropathological biomarkers associated with transporters of the blood-brain barrier (BBB). Despite the well-characterized clinical indication of decreasing Aβ levels in the cerebrospinal fluid (CSF) during the development of Alzheimer's disease (AD), the link between the alternation of Aβ level in the blood and the progress of the disorder is still controversial. Here, we report a direct correlation of Aβ(1-42) levels between CSF and plasma in AD mouse model. We injected monomeric Aβ(1-42) directly into the intracerebroventricular (ICV) region of normal adult mouse brains to induce AD-like phenotypes. Using sandwich enzyme-linked immunosorbent assays, we observed proportional elevation of Aβ(1-42) levels in both CSF and plasma in a dose-dependent manner. Our findings that plasma Aβ(1-42) reflects the condition of CSF Aβ(1-42) warrant further investigation as a biomarker for the blood diagnosis of AD.

MATERIALS
Product Number
Brand
Product Description

USP
Valacyclovir Related Compound G, United States Pharmacopeia (USP) Reference Standard
Valaciclovir impurity G, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
Piperidine, biotech. grade, ≥99.5%
Sigma-Aldrich
Fmoc-Asp(OtBu)-OH, ≥98.0% (HPLC)
Sigma-Aldrich
Piperidine, ReagentPlus®, 99%
Sigma-Aldrich
N-Ethyldiisopropylamine solution, suitable for peptide synthesis, ~2 M in 1-methyl-2-pyrrolidinone
Sigma-Aldrich
N-Ethyldiisopropylamine, BASF quality, ≥98.0%
Sigma-Aldrich
Piperidine, ≥99.5%, purified by redistillation
Sigma-Aldrich
Fmoc-Ser(tBu)-OH, ≥98.0% (HPLC)
Sigma-Aldrich
Fmoc-Arg(Pbf)-OH, ≥98.0% (HPLC)
Sigma-Aldrich
Piperidine, SAJ first grade, ≥99.0%
Sigma-Aldrich
Fmoc-Phe-OH, 98%
Sigma-Aldrich
Fmoc-Gln(Trt)-OH, ≥98.0% (HPLC)
Sigma-Aldrich
Fmoc-Leu-OH, ≥97.0%
Sigma-Aldrich
Fmoc-Met-OH, ≥98.0% (HPLC)
Sigma-Aldrich
Fmoc-Lys(Boc)-OH, ≥98.0% (HPLC)
Sigma-Aldrich
Fmoc-Glu(OtBu)-OH, ≥98.0% (HPLC)
Sigma-Aldrich
Fmoc-Ala-OH, 95%
Sigma-Aldrich
N,N-Diisopropylethylamine, ReagentPlus®, ≥99%
Sigma-Aldrich
4-(Dimethylamino)pyridine, purum, ≥98.0% (NT)
Supelco
Anisole, analytical standard
Supelco
Anisole, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
4-(Dimethylamino)pyridine, ReagentPlus®, ≥99%
Sigma-Aldrich
Ethylenediaminetetraacetic acid, 99.995% trace metals basis
Sigma-Aldrich
Piperidine solution, suitable for peptide synthesis, 20% in DMF
Sigma-Aldrich
Triisopropylsilane, 98%
Sigma-Aldrich
Anisole, anhydrous, 99.7%
Sigma-Aldrich
Anisole, ReagentPlus®, 99%
Sigma-Aldrich
Ethylenediaminetetraacetic acid, BioUltra, ≥99.0% (KT)
Sigma-Aldrich
Anisole, ≥99%, FCC, FG