Skip to Content
MilliporeSigma
  • Correlation of 3-mercaptopropionic acid induced seizures and changes in striatal neurotransmitters monitored by microdialysis.

Correlation of 3-mercaptopropionic acid induced seizures and changes in striatal neurotransmitters monitored by microdialysis.

European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences (2014-01-28)
Eric W Crick, Ivan Osorio, Mark Frei, Andrew P Mayer, Craig E Lunte
ABSTRACT

The goal of this study was to use a status epilepticus steady-state chemical model in rats using the convulsant, 3-mercaptopropionic acid (3-MPA), and to compare the changes in striatal neurotransmission on a slow (5min) and fast (60s) timescale. In vivo microdialysis was combined with electrophysiological methods in order to provide a complete evaluation of the dynamics of the results obtained. To compare the effects of a steady-state chemical model pof status epilepticus on striatal amino-acid and amine neurotransmitters contents, as measured via in vivo microdialysis combined with electrophysiological methods. Measurements were performed on samples collected every 60s and every 5min. "Fast" (60s) and "slow" (5min) sampling timescales were selected, to gain more insight into the dynamics of GABA synthesis inhibition and of its effects on other neurotransmitters and on cortical electrical activity. 3-MPA was administered in the form of an intra-venous load (60mg/kg) followed by a constant infusion (50mg/kg/min) for min. Microdialysis samples were collected from the striatum at intervals of 5min and 60s and analyzed for biogenic amine and amino acid neurotransmitters. ECoG activity was monitored via screws placed over the cortex. In the 5min samples, glutamate (Glu) increased and γ-aminobutyric acid (GABA) decreased monotonically while changes in dopamine (DA) concentration were bimodal. In the sixty second samples, Glu changes were bimodal, a feature that was not apparent with the 5min samples. ECoG activity was indicative of status epilepticus. This study describes the combination of in vivo microdialysis with electrophysiology to monitor the effect of 3-MPA on neurotransmission in the brain. This led to a better understanding of the chemical changes in the striatum due to the applied 3-MPA chemical model of status epilepticus.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Methanol, suitable for HPLC
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetonitrile solution, contains 10.0% acetone, 0.05% formic acid, 40.0% 2-propanol
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium hydroxide solution, 0.05 M
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium hydroxide solution, 6 M
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Methanol, SAJ first grade, ≥99.5%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Methanol, JIS 300, ≥99.8%, for residue analysis
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium hydroxide solution, 1 M
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium hydroxide solution, 0.01 M
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium hydroxide solution, 7 M
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Ammonium acetate solution, 50 % (w/v), for copper determination
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium hydroxide solution, 0.1 M
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium hydroxide solution, 4 M
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium hydroxide, JIS special grade, ≥96.0%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Methanol, JIS special grade, ≥99.8%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Methanol, NMR reference standard
SKU
Pack Size
Availability
Price
Quantity
Supelco
L-Aspartic acid, Pharmaceutical Secondary Standard; Certified Reference Material
SKU
Pack Size
Availability
Price
Quantity
Supelco
Dopamine hydrochloride, Pharmaceutical Secondary Standard; Certified Reference Material
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetonitrile solution, contains 0.05 % (w/v) ammonium formate, 5 % (v/v) water, 0.1 % (v/v) formic acid, suitable for HPLC
SKU
Pack Size
Availability
Price
Quantity
Dopamine hydrochloride, European Pharmacopoeia (EP) Reference Standard
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetonitrile solution, contains 0.05 % (v/v) trifluoroacetic acid
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetonitrile solution, contains 0.1 % (v/v) trifluoroacetic acid, suitable for HPLC
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium hydroxide, ultra dry, powder or crystals, 99.99% trace metals basis
SKU
Pack Size
Availability
Price
Quantity
Trimethyltetradecylammonium bromide, European Pharmacopoeia (EP) Reference Standard
SKU
Pack Size
Availability
Price
Quantity
Supelco
L-Aspartic acid, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Methanol, HPLC Plus, ≥99.9%, poly-coated bottles
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
3-Ethyl-2,4-pentanedione, mixture of tautomers, 98%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Methanol, anhydrous, 99.8%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
3,4-Dihydroxyphenylacetic acid, 98%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Ammonium acetate, 99.999% trace metals basis
SKU
Pack Size
Availability
Price
Quantity
Supelco
Sodium hydroxide solution, 49-51% in water, eluent for IC
SKU
Pack Size
Availability
Price
Quantity