Skip to Content
MilliporeSigma
  • Teicoplanin bonded sub-2 μm superficially porous particles for enantioseparation of native amino acids.

Teicoplanin bonded sub-2 μm superficially porous particles for enantioseparation of native amino acids.

Journal of pharmaceutical and biomedical analysis (2015-06-16)
Yi Min, Zhigang Sui, Zhen Liang, Lihua Zhang, Yukui Zhang
ABSTRACT

Superficially porous particles (SPPs) demonstrate superior efficiency than totally porous particles in chiral separations. In order to obtain high efficiency and fast separation, sub-2 μm SPPs with high surface area are synthesized, and with teicoplanin bonded, such materials are successfully applied into the rapid enantioseparation of native amino acids. In brief, 1.27 ± 0.06 μm nonporous silica particles are prepared by a modified seeded growth method, followed by mesoporous shell fabrication via one-pot templated dissolution and redeposition strategy, and pore size expansion via acid-refluxing. The diameter of the formed SPPs is 1.49 ± 0.04 μm, with the shell thickness as 206 nm. Nitrogen physisorption experiments show that the Brunauer-Emmett-Teller (BET) specific surface area is 213.6 m(2)/g and pore size is 9 nm. After teicoplanin derivatization with bonding capacity as 83.3 μmol/g, the prepared chiral stationary phase is packed into a stainless steel tube with the geometry of 50 mm × 2.1 mm i.d.. In less than 6.4 min, six native amino acids (norleucine, alanine, valine, methionine, leucine, norvaline) are enantioseparated with resolution factors ranging from 1.9 to 5.0. Besides, the resolution for chiral separation is improved with ethanol-water instead of methanol-water as the mobile phase. Moreover, the low temperature gives higher resolution, but longer retention time and higher backpressure. Finally, the effect of flow rate on enantiomeric separation is studied and fast chiral separation within 1 min is obtained with flow rate of 0.4 mL/min. All these results show that the synthesized teicoplanin bonded sub-2 μm SPPs have great potential to achieve the enantioseparation of native amino acids with high resolution and rapid speed.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Ammonium fluoride, ≥99.99% trace metals basis
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Tridecane, ≥99%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Hexadecyltrimethylammonium chloride, ≥98.0% (NT)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetic acid, for luminescence, BioUltra, ≥99.5% (GC)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetic acid, natural, ≥99.5%, FG
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetic acid, ≥99.5%, FCC, FG
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Chloroform, ACS reagent, ≥99.8%, contains amylenes as stabilizer
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Cetyltrimethylammonium chloride solution, 25 wt. % in H2O
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Chloroform, anhydrous, ≥99%, contains 0.5-1.0% ethanol as stabilizer
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Chloroform, anhydrous, contains amylenes as stabilizer, ≥99%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Chloroform, ACS reagent, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer
SKU
Pack Size
Availability
Price
Quantity
Supelco
Chloroform, suitable for HPLC, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
DL-Norleucine
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Adenosine 5′-phosphosulfate sodium salt, ≥85%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Chloroform, ≥99%, PCR Reagent, contains amylenes as stabilizer
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetic acid, SAJ first grade, ≥99.0%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetic acid, ≥99.7%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetic acid, ≥99.7%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetic acid, JIS special grade, ≥99.7%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetic acid, 99.5-100.0%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetic acid solution, 1 M, 1 N
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetic acid, ≥99.7%, suitable for amino acid analysis
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Chloroform, suitable for HPLC
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetonitrile solution, contains 10.0% acetone, 0.05% formic acid, 40.0% 2-propanol
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Chloroform, SAJ first grade, ≥99.0%, contains 0.4-0.8% ethanol
SKU
Pack Size
Availability
Price
Quantity