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  • Glucose oxidase adsorption performance of carbonaceous mesocellular foams prepared with different carbon sources.

Glucose oxidase adsorption performance of carbonaceous mesocellular foams prepared with different carbon sources.

Journal of bioscience and bioengineering (2014-12-31)
Fengjiao Zhou, Ximing Pu, Dapeng Luo, Guangfu Yin, Kai Zhuang, Xiaoming Liao, Zhongbin Huang, Xianchun Chen, Yadong Yao
ABSTRACT

Several carbonaceous mesocellular foams (C-MCFs) were prepared with MCF-silica as template using the carbon precursors of sucrose, furfuryl alcohol and lab-made phenolic resin, and the corresponding C-MCFs were named as C-MCF-Suc, C-MCF-FA and C-MCF-PR, respectively. The results of SEM, transmission electron microscopy, N2 adsorption-desorption and energy-dispersive X-ray measurements indicated that the C-MCFs prepared from different carbon source appeared morphologically with different degree of order and different pore distribution. The C-MCF-FA exhibited the highest ordered structure and the smallest pore distribution among the foams. The optimum conditions for adsorption of C-MCFs on glucose oxidase (GOD) were also studied, and the maximum adsorbance was determined. The adsorption of GOD on C-MCF-FA was performed at different pH with different GOD concentrations. The maximum adsorption (423.3 mg g(-1)) was observed near the isoelectric point of the GOD (pI ≈ 5.0) with a GOD concentration of 6.0 mg mL(-1), suggesting that the GOD adsorption on C-MCFs might be affected strongly by the electric repulsion between the GOD molecules. Moreover, GOD adsorption performances on different C-MCFs revealed that both the pore size and the pore volume played important roles in the adsorption process, and the window size of C-MCFs dominated the residual immobilized amounts of GOD. Compared to the other two C-MCFs, the C-MCF-FA with a smaller window pore (10 nm) and higher volume (1.40 cm(3) g(-1)) exhibited the highest GOD adsorption and catalytic activity. Furthermore, the immobilized GOD exhibited improved thermal and storable stabilities. Thus the C-MCF-FA could be served as the prospective GOD carrier material used in enzymatic fuel cells.

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o-Dianisidine dihydrochloride, Suitable for use in glucose determination
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Propylene glycol, ≥99.5% (GC), FCC, FG
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3,3′,5,5′-Tetramethylbenzidine, ≥99%
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Ethylene glycol, JIS special grade, ≥99.5%
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Ethylene glycol, SAJ first grade, ≥99.0%
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Ethylene glycol solution, NMR reference standard, 80% in DMSO-d6 (99.9 atom % D), NMR tube size 5 mm × 8 in.
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3,3′,5,5′-Tetramethylbenzidine, tablet, 1 mg substrate per tablet
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o-Dianisidine dihydrochloride, tablet, 10 mg substrate per tablet
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Propylene Glycol, meets USP testing specifications
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Ethylene glycol, BioUltra, ≥99.5% (GC)
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Sucrose, ≥99.5% (GC), Grade II, suitable for plant cell culture
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Sucrose, meets USP testing specifications
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Sucrose, BioUltra, for molecular biology, ≥99.5% (HPLC)
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1,2-Propanediol, puriss. p.a., ACS reagent, ≥99.5% (GC)
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Furfuryl alcohol, 98%
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1,2-Propanediol, ACS reagent, ≥99.5%
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