05-117

Anti-FGF-2/basic FGF (neutralizing) Antibody, clone bFM-1

clone bFM-1, Upstate^®, from mouse

• 別名: Basic fibroblast growth factor, basic fibroblast growth factor bFGF, fibroblast growth factor 2, fibroblast growth factor 2 (basic), heparin-binding growth factor 2., GSK3 beta, GSK3B, Glycogen synthase kinase 3 beta, Glycogen synthase kinase 3β
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• クローン: bFM-1, monoclonal
• application: Neutral; RIA
• 化学種の反応性: human, mouse, bovine, rat
• テクニック: neutralization: suitable; radioimmunoassay: suitable
• citations: 56

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: bFM-1, monoclonal
• フォーム: liquid
• 分子量: 17.5 kDa
• 化学種の反応性: human, mouse, bovine, rat
• メーカー／製品名: Upstate^®
• テクニック: neutralization: suitable; radioimmunoassay: suitable
• アイソタイプ: IgG1κ
• 適合性: not suitable for Western blot
• NCBIアクセッション番号: NM_002006.3
• UniProtアクセッション番号: P09038
• 輸送温度: dry ice
• 遺伝子情報: human ... FGF2(2247) ; mouse ... Fgf2(281161) , Fgf2(14173) ; rat ... Fgf2(54250)

詳細

詳細

Fibroblast growth factor 2 (FGF-2) belongs to the family of heparin-binding growth factors. It is localized in the cells of the mesoderm and neuroectodermal origin and several tumor cells. FGF-2 exists in four isoforms. It is made up of four cysteine residues, many basic residues, and two active sites. FGF-2 protein is a trigonal pyramidal structure formed by twelve anti-parallel β-sheets. The FGF-2 chromosome is located on the human chromosome at 4q28.1.

Research area: Cancer

特異性

This antibody is highly specific for the non-denatured form of FGF-2/basic FGF from bovine, human, rat and mouse sources. Does not cross-react with bovine acidic FGF.

This antibody recognizes the non-denatured form of FGF-2/basic FGF; does not cross-react with bovine acidic FGF.

免疫原

Epitope: a.a. 5-24

Purified bovine brain basic FGF (HBGF-2). Clone bFM-1.

アプリケーション

Anti-FGF-2/basic FGF (neutralizing) Antibody, clone bFM-1 has been used in:

• FGF2 neutralization assay (6 or 12 µg/mL)
• in vitro tube formation assay (1000 ng/mL)
• immunolabelling (1:200)

生物化学的／生理学的作用

Fibroblast growth factor 2 (FGF-2) plays a role in vertebrate embryonic and fetal development. It acts as an inducer of angiogenesis and is a well-known mitogen (in vitro) for a variety of cells including fibroblasts and vascular endothelial cells. FGF-2 exerts its biological activity through high-affinity tyrosine kinase receptors and low-affinity heparan sulfate proteoglycans present on the cell surface. It exhibits autocrine effects on various cell functions involved in angiogenesis such as proliferation, integrin expression, migration, and proteinase production. FGF-2 participates in the growth, migration, differentiation, and survival of a variety of cells. The binding of FGF-2 with heparin protects FGF-2 from denaturation by heat and acid and proteolytic cleavage.

品質

Routinely evaluated by neutralization of greater than 50% of growth of FBHE cells simulated with FGF-2/bFGF.

Neutralization of bFGF Activity Assay:
2.5 µg/mL of this lot of antibody neutralized greater than 40% of growth of FBHE cells simulated with 5 ng/mL of FGF-2/bFGF.

ターゲットの説明

17.5 kDa

物理的形状

Ammonium sulfate precipitation and DEAE-cellulose chromatography

Format: Purified

Purified mouse monoclonal IgG1κ in TBS. Protein determined by a Bradford microtiter assay using mouse IgG as the standard. Frozen solution.

保管および安定性

Stable for 1 year at -20ºC from date of receipt. Aliquot to avoid repeated freezing and thawing. For maximum recovery of the product, centrifuge the original vial after thawing and prior to removing the cap.

アナリシスノート

Control
Fetal liver or kidney tissue.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable