05-1923

Anti-phospho-Threonine Antibody, clone 20H6.1

clone 20H6.1, from mouse

• 別名: Threonine
• UNSPSCコード: 12352203
• eCl@ss: 32160702

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品タイプ: primary antibodies
• クローン: 20H6.1, monoclonal
• 化学種の反応性: mouse, human
• 化学種の反応性（ホモロジーによる予測）: vertebrates (based on 100% sequence homology), all
• テクニック: immunocytochemistry: suitable; western blot: suitable
• アイソタイプ: IgG2bκ
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: phosphorylation (pThr)

詳細

詳細

Protein phosphorylation is normally associated with protein modification in signaling pathways and may be useful in pathophysiology and treatment of major neuropsychiatric disorders. Antibodies against phospho-threonine have been used to establish stability of the phosphorylated forms of proteins that may be involved in many different types of signaling. Phospho-threonine antibody is used to measure phosphorylation levels of PSII reaction center proteins D1, D3 and chlorophyll a binding protein CP43.

免疫原

KLH-conjugated linear peptide corresponding to Threonine.

アプリケーション

Research Category
細胞シグナル伝達

Research Sub Category
一般的な翻訳後修飾

Immunoprecipitation Analysis: 10 µg of this antibody immunoprecipitated Threonine from 100 µg of A431 cells stimulated with calyculin/okadaic acid.

Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected Threonine in A431, NIH/3T3, and HeLa cells.

This phospho-Threonine antibody is validated for use in WB & ICC for the detection of the phospho-Threonine protein.

品質

Evaluated by Western Blot in calyculin/okadaic acid treated and untreated A431 cell lysates.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Threonine on 10 µg of calyculin/okadaic acid treated and untreated A431 cell lysates.

ターゲットの説明

Various observed

物理的形状

Protein G

Format: Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

アナリシスノート

Control
Calyculin/okadaic acid treated and untreated A431 cell lysates.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable