391340

HEPES

≥99% (alkalimetric), solid, pH 6.8-8.2, Molecular Biology

• 別名: 4-(2-ヒドロキシエチル)ピペラジン-1-エタンスルホン酸, N-(2-ヒドロキシエチル)ピペラジン-N′-(2-エタンスルホン酸)
• 実験式（ヒル表記法）: C₈H₁₈N₂O₄S
• CAS番号: 7365-45-9
• 分子量: 238.30
• Beilstein: 883043
• MDL番号: MFCD00006158
• UNSPSCコード: 12161704
• NACRES: NA.25

特性

• 製品名: HEPES, A zwitterionice N-substituted aminosulfonic acid buffer. Useful in the pH 6.8-8.2 range. Has a pKa of 7.48 at 25°C.
• グレード: Molecular Biology
• 品質水準: 100
• アッセイ: ≥99% (alkalimetric)
• フォーム: solid
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze
• 色: white
• 有効pH範囲: 6.8-8.2
• pKa (25 °C): 7.5
• 溶解性: water: 400 mg/mL; aqueous base: soluble
• 微量陽イオン: heavy metals: ≤0.0005%
• その他の活性: DNase, RNase, and protease, none detected
• 輸送温度: ambient
• 保管温度: 15-25°C
• SMILES記法: OCCN1CCN(CC1)CCS(O)(=O)=O
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N

詳細

詳細

A zwitterionice N-substituted aminosulfonic acid buffer. Useful in the pH 6.8-8.2 range. Has a pK_a of 7.48 at 25°C.

アプリケーション

• Fluorescent labeling of the root cap cells with the bioactive NBD-S chemical probe based on the cellulose biosynthesis inhibition herbicides: This innovative study demonstrates the application of molecular biology grade HEPES in the formulation of bioactive probes for the fluorescent labeling of root cap cells. Using cellulose biosynthesis inhibition herbicides as a model system, this research provides valuable insights into plant biology, aiding in the understanding of plant cell wall structures and functions. The use of HEPES helps maintain the physiological pH during the experiments, ensuring the stability and effectiveness of the chemical probes used (Tamogami S et al., 2021).

警告

Toxicity: Standard Handling (A)

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 13 - Non Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable