861006

エオシンB

certified by the Biological Stain Commission, Dye content 90 %

• 別名: 4′,5′-ジブロモ-2′,7′-ジニトロフルオレセイン 二ナトリウム塩, アシッドレッド91, エオシンスカーレット, エオシンブルーイッシュ, サフロシン
• 化学式: C₂₀H₆N₂O₉Br₂Na₂
• CAS番号: 548-24-3
• 分子量: 624.06
• カラーインデックス番号: 45400
• Beilstein: 4121964
• EC Number: 208-943-1
• MDL番号: MFCD00005041
• UNSPSCコード: 12171500
• PubChem Substance ID: 24888525
• NACRES: NA.47

特性

• グレード: certified by the Biological Stain Commission
• 品質水準: 200
• フォーム: powder
• 組成: Dye content, 90%
• テクニック: microbe id | staining: suitable
• 溶解性: H₂O: 1 mg/mL, clear, red
• λmax: 395 nm; 514 nm
• アプリケーション: diagnostic assay manufacturing; hematology; histology
• 保管温度: room temp
• SMILES記法: [Na+].[Na+].[O-]C(=O)c1ccccc1C2=C3C=C(C(=O)C(Br)=C3Oc4c(Br)c([O-])c(cc24)[N+]([O-])=O)[N+]([O-])=O
• InChI: 1S/C20H8Br2N2O9.2Na/c21-14-16(25)11(23(29)30)5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(24(31)32)17(26)15(22)19(10)33-18(9)14;;/h1-6,25H,(H,27,28);;/q;2*+1/p-2
• InChI Key: GYYTYUGGVBYJHE-UHFFFAOYSA-L

詳細

アプリケーション

• Eosin B has been used for the histological analysis of anal sphincter sections obtained from rats.
• It has been used along with Mayer′s hematoxylin for the staining of triangularis sterni muscle sections.
• It has been used along with hematoxylin for the staining of different tissue sections obtained from mouse.

生物化学的／生理学的作用

Eosin B is a xanthene dye and is also referred to as eosin bluish or erythrosin B. It is used in the Harris stain for Negri bodies. Eosin B is also used in Mayer′s Hemalum as a counterstain for collagen. Eosin B-Azure A are used together for the staining of cell granules, nuclei and microorganisms. It can also be used for protein estimation. The protein - eosin B complex absorbs at wavelength range of 536–544nm and the change in blue shift helps in determining the protein concentration.

適合性

Mayerのヘマラム染色後のコラーゲンの対比染色剤です。 細胞顆粒や核、微生物の組織染色剤としてアズールAとともに用いられます。下垂体前葉の細胞分染に用いられます。

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, type N95 (US)