A8967

抗アミロイド前駆体タンパク質, N-末端 ウサギ宿主抗体

IgG fraction of antiserum, buffered aqueous solution

• 別名: 抗APP抗体
• MDL番号: MFCD02095830
• UNSPSCコード: 12352203
• NACRES: NA.41
• 結合体: unconjugated
• application: ARR; IHC (p); WB
• クローン: polyclonal
• 化学種の反応性: human, rat, mouse
• citations: 23
• テクニック: immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using formic acid-treated sections of human Alzheimer′s disease (AD) brain; microarray: suitable; western blot: 1:1,000 using rat brain extract or supernatant of 293T cells secreting APP.

特性

• 由来生物: rabbit
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: IgG fraction of antiserum
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• フォーム: buffered aqueous solution
• 分子量: antigen 95-100 kDa
• 化学種の反応性: human, rat, mouse
• テクニック: immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using formic acid-treated sections of human Alzheimer′s disease (AD) brain; microarray: suitable; western blot: 1:1,000 using rat brain extract or supernatant of 293T cells secreting APP.
• UniProtアクセッション番号: P05067
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... APP(351) ; mouse ... App(11820) ; rat ... App(54226)

詳細

詳細

免疫原の配列は、APP₇₅₁ およびAPP₇₇₀のAPPアイソフォ-ムと同一であり、ラット・マウスのAPP₆₉₅で高度に保存されています(1アミノ酸置換)。本抗体は、APP₆₉₅, APP₇₅₁, APP₇₇₀を認識します。

Amyloid precursor proteins (APPs) are transmembrane glycoproteins that are found in a wide range of tissues. APPs have 3 main isoforms, namely, APP695, APP751 and APP770 that are derived from alternative splicing events in cells. It is expressed at high levels in the brain. APP gene is mapped to human chromosome 21q11.2-q21. It is a 695 amino acid protein which possesses a large ectodomain and comparatively short intracellular region.

免疫原

ヒトAPP₆₉₅のN末端(アミノ酸46-60)に相当する合成ペプチドのKLH結合体。

アプリケーション

Anti-Amyloid Precursor Protein, N-Terminal antibody produced in rabbit has been used in:

• western blotting
• immunostaining
• immunofluorescence

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

SH-SY5Y cell lysates were analyzed by western blot using rabbit anti-Amyloid Precursor Protein, C-Terminal as the primary antibody at a 1:500 dilution.

生物化学的／生理学的作用

Amyloid precursor proteins (APPs) regulates cell growth, motility, neurite outgrowth and cell survival. The intracellular C-terminus of APP serves as a transcriptional regulator and as a receptor for kinesin-1-mediated axonal transport. Alzheimer′s disease is characterized by deposition of amyloid in the central nervous system, in neurite plaques and on cerebral vasculature. Mutations in the APP gene are linked with rare forms of autosomal dominant familial Alzheimer′s Disease (FAD). APPs undergo post-translational processing including N- and O-glycosylation, phosphorylation and sulfation.

物理的形状

0.01M PBS溶液 (pH 7.4, 15mMアジ化ナトリウム含有)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable