F2043

モノクロナール抗β-チューブリン−FITC マウス宿主抗体

clone TUB 2.1, purified from hybridoma cell culture

• 別名: モノクロナール抗β-チューブリン
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: FITC conjugate
• 抗体製品の状態: purified from hybridoma cell culture
• 抗体製品タイプ: primary antibodies
• クローン: TUB 2.1, monoclonal
• 形状: buffered aqueous solution
• 分子量: antigen 55 kDa
• 化学種の反応性: human, rat, frog, moth, mouse, plant, rabbit, chicken, bovine, wheat, sea urchin, hamster
• 保管条件: protect from light
• テクニック: direct immunofluorescence: 1:25 using cultured BHK cells
• アイソタイプ: IgG1
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... TUBB(203068) , TUBB1(81027) , TUBB1(81027) , TUBB1(81027) , TUBB1(81027) , TUBB2A(7280) , TUBB2A(7280) , TUBB2A(7280) , TUBB2A(7280) , TUBB2C(10383) , TUBB2C(10383) , TUBB2C(10383) , TUBB2C(10383) ; mouse ... Tubb1(104068) , Tubb1(104068) , Tubb1(104068) , Tubb1(104068) , Tubb2a(22151) , Tubb2a(22151) , Tubb2a(22151) , Tubb2a(22151) , Tubb2c(227613) , Tubb2c(227613) , Tubb2c(227613) , Tubb2c(227613) , Tubb3(22152) ; rat ... Tubb2(29212) , Tubb2(29212) , Tubb2(29212) , Tubb2(29212) , Tubb2c(296554) , Tubb2c(296554) , Tubb2c(296554) , Tubb2c(296554)

詳細

詳細

β-Tubulin belongs to the subfamily of tubulins. It has two β-sheets enclosed by α-helices. It also contains an N-terminal domain with the guanine nucleotide-binding region, an intermediate domain with taxol-binding site and a C-terminal domain, which has the binding surface for molecular motor proteins. Human β-tubulin consists of seven isoforms (βI (class I), βII (class II), βIII (class III), βIVa (class IVa), βIVb (class IVb), βV (class V), and βVI (class VI)). TUBB1 (tubulin beta class I) is located on human chromosome 6p21.33.(10)

Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton. Tubulin is a heterodimer, which consists of α-tubulin and β-tubulin; both subunits have a molecular weight of 55 kDa and share considerable homology. The most studied tubulins have been isolated from vertebrate brains. The microtubules can be viewed in immunofluorescent microscopy, which enables the observation of the intracellular organization of proteins that are in the form of a supramolecular structure.

特異性

抗体はβ-チュ-ブリンの5種類のアイソフォ-ム(β1-β5)をすべて認識します。イムノブロッティングでは、β-チュ-ブリンのC末端部分のβ-Lcおよびβ-Scフラグメントと反応します。抗体は、培養細胞や組織切片のβ-チュ-ブリンの特定に使用することができます。

免疫原

ラット脳由来チュ-ブリン。

アプリケーション

FITC-β tubulin antibody may be used for detection ajnd localization of β-tubulin by direct labeling in a variety of eukaryotic cells ranging from human embryonic kidney cells (HEK293T) to cells of azuki bean epicotyl. by immunohistochemical and immunocytochemical assays.

Monoclonal Anti-β-Tubulin-FITC antibody has been used:

• in the evaluation of the status of the meiotic spindle and the arrangement of chromosomes
• in immunofluorescence studies
• in immunofluorescence microscopy
• in spindle and chromosome imagery(6)

生物化学的／生理学的作用

Mutation in β-Tubulin gene leads to various neuronal migration disorders such as lissencephaly, pachygyria and polymicrogyria malformations. β-1 tubulin, also known as class VI or TUBB1, plays a vital role in platelet production and is considered to be a potential biomarker candidate for immune thrombocytopenia (ITP).

物理的形状

0.01M PBS溶液(pH 7.4, 1%BSA, 15mMアジ化ナトリウム含有)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable