L3272

ルリアアガーベース(ミラー改変)

Powder microbial growth medium

• 別名: Luria Agar Base (Miller’s Modification)
• UNSPSCコード: 41106200
• NACRES: NA.85

特性

• グレード: for molecular biology
• 品質水準: 200
• 無菌性: non-sterile
• 形状: powder
• 組成: Agar, 15 g/L ; NaCl, 0.5 g/L ; Tryptone, 10 g/L ; Yeast Extract, 5 g/L
• テクニック: microbiological culture: suitable
• pH: 6.8-7.2(3.05% solution)
• アプリケーション: food and beverages; microbiology
• 保管温度: room temp
• 適合性: nonselective for Escherichia coli; nonselective for coliforms

詳細

詳細

Luria′s low salt LB is a microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates. The addition of agar provides a solid medium for microbial growth.

アプリケーション

LB Broth with agar (Luria low salt) has been used:

• for culturing E. coli DH10B cells.
• in the isolation of diazotrophic bacteria.
• for transformation of competent E. coli strain Stbl3 for cloning.

Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin^®.

特徴および利点

Luria′s low salt LB powder with agar provides:

• Easy scale-up using larger package sizes
• A budget-friendly alternative to pre-poured plates
• Standard formulation

調製ノート

1. Suspend 30.5 g in 1 L of distilled water.
2. Heat to boiling while stirring to dissolve.
3. Autoclave for 15 minutes at 121°C.
4. Cool to 50°C prior to dispensing into petri dishes.

法的情報

Zeocin is a registered trademark of Cayla Sarl

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable