SAE0049

Lactic Dehydrogenase, recombinant 大腸菌由来

from human, recombinant, expressed in E. coli, aqueous solution

• 別名: (S)-ラクテート：NAD⁺オキシドレダクターゼ, L-ラクテートデヒドロゲナーゼ, 乳酸
• CAS番号: 9001-60-9
• Enzyme Commission number: 1.1.1.27 (BRENDA, IUBMB)
• MDL番号: MFCD00131466
• UNSPSCコード: 12352200
• NACRES: NA.54

特性

• 由来生物: human
• 品質水準: 200
• リコンビナント: expressed in E. coli
• 形状: aqueous solution
• 保管条件: (Keep container tightly closed in a dry and well-ventilated place)
• 色: colorless
• UniProtアクセッション番号: K1T0A2
• 輸送温度: dry ice
• 保管温度: −20°C
• 遺伝子情報: human ... LDHA(3939)

詳細

詳細

Research area: Cell Signaling
The gene LDHA (L-lactate dehydrogenase A chain) is mapped to human chromosome 11p15. It is a subunit of lactate dehydrogenase.In particular, lactic dehydrogenase A (LDHA) is mainly found in skeletal muscle, and for that reason is known as the M subunit. This recombinant form of LDHA has a C-terminal histidine-tag.

アプリケーション

L-Lactate Dehydrogenase (LDHA) has been used in in vitro phosphoglycerate mutase 1 (PGAM1) inhibitors screening assay. It has also been used in a colorimetric assay for determining lactate concentration in conditioned media.

生物化学的／生理学的作用

L-lactic dehydrogenase catalyzes the conversion of L-lactate into L-pyruvate while reducing NAD⁺ to NADH and H⁺.  L-lactate dehydrogenase A chain (LDHA), an enzyme involved in pyruvate metabolism, LDH is responsible for the conversion of pyruvate to lactate, the final step of glycolysis. It is overexpressed in various cancers. LDHA regulates the microenvironment of developing tumors by the hypoxia-inducible factor (HIF)-signaling pathway. LDHA aids in the NAD⁺ regeneration during the β-oxidation of fatty acid. LDHA (L-lactate dehydrogenase A chain) is responsible for the conversion of pyruvate to lactate, the final step of glycolysis. It is overexpressed in various cancers. In cancer cells, HIF-1a (hypoxia-inducible factor) induces the expression of LDHA, which helps in maintaining glycolysis in cells.

L-乳酸デヒドロゲナーゼは、L-乳酸をL-ピルビン酸に変換する反応を触媒しながら、NAD⁺を還元してNADHおよびH⁺を生成します。

単位の定義

One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.

物理的形状

Buffered aqueous solution with Hepes (pH 7.5), NaCl and glycerol.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable