C6423

α-Chymotrypsin from bovine pancreas

suitable for protein sequencing, salt-free, lyophilized powder

• CAS Number: 9004-07-3
• 효소 위원회 번호: 3.4.21.1 (BRENDA, IUBMB)
• EC Number: 232-671-2
• MDL number: MFCD00130481
• UNSPSC 코드: 12352204
• eCl@ss: 42010112
• NACRES: NA.26

속성

• Grade: Proteomics Grade
• Quality Level: 200
• 양식: salt-free, lyophilized powder
• 분자량: 25 kDa
• 적합성: suitable for protein sequencing
• UniProt 수납 번호: P00767
• 저장 온도: 2-8°C
• 유전자 정보: cow ... CTRB1(618826)

설명

일반 설명

α-Chymotrypsin from bovine pancreas (bovine pancreatic α-chymotrypsin, CHT) is an enzyme protein. The influence of varying concentrations of organic solvents like ethanol, 1,4-dioxane and acetonitrile on CHT has been reported.

Chymotrypsin (Chy) is a serine protease. It corresponds to a molecular weight of 25.7 kDa and is widely used in pharmaceutical industry. It is synthesized in pancreas from chymotrypsinogen and require calcium for this conversion.

애플리케이션

α-Chymotrypsin from bovine pancreas is used for the following applications:

• Protein Identification by mass spectrometry (MS)
• The isolation and characterization of myosin heavy chains
• Toxin preparation
• The incubation of infected and uninfected cells for analysis of cellular proteins by SDS-PAGE

α-Chymotrypsin from bovine pancreas (BPC) may be used as a catalyst in the preparation of tetrahydroquinoline derivatives by Povarov reaction.

생화학적/생리학적 작용

α-Chymotrypsin from bovine pancreas is stabilized by Ca²⁺ and catalyzes the hydrolysis of peptide bonds in particular the amino acids tyrosine, phenylalanine, tryptophan, and leucine at their C-terminal side. α-Chymotrypsin is inhibited by Cu²⁺ and Hg.

A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met, Leu) on the carboxyl end of the bond.

단위 정의

One unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.

기타 정보

View more information on chymotrypsin at www.sigma-aldrich.com/enzymeexplorer

안전성 정보

• 픽토그램: GHS08,GHS07,GHS09
• 신호어: Danger
• 유해 및 위험 성명서: H302,H315,H319,H334,H335,H400
• 예방조치 성명서: P261 - P264 - P273 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• 표적 기관: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• 개인 보호 장비: dust mask type N95 (US), Eyeshields, Faceshields, Gloves