CHY5S

α-Chymotrypsin from bovine pancreas

≥40 units/mg protein, vial of 5 mg

• 동의어(들): α-chymotrypsin A and B
• CAS Number: 9004-07-3
• 효소 위원회 번호: 3.4.21.1 (BRENDA, IUBMB)
• EC Number: 232-671-2
• MDL number: MFCD00130481
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: bovine pancreas
• Quality Level: 200
• 설명: aseptically filled
• 유형: Type IV-S
• 양식: solid
• 특이 활성도: ≥40 units/mg protein
• 분자량: 25 kDa
• 구성: protein, ≥85% UV
• 포장: vial of 5 mg
• UniProt 수납 번호: P00767
• 저장 온도: −20°C
• 유전자 정보: cow ... CTRB1(618826)

설명

애플리케이션

α-Chymotrypsin from bovine has been used in a study to inform proteasome inhibition in order to advance anticancer research. α-Chymotrypsin from bovine has also been used in a study that functionalized surface anchored poly(methylhydrosiloxane) thin films on oxidized silicon wafers.

α-Chymotrypsin from bovine pancreas has been used:

• as a supplement for the collection of semen into Tris diluent
• as one of the proteases in the analysis of major histocompatibility complex (MHC) class II protease sensitivity assay
• as a component in YEPD broth for biofilm dispersion assay
• in the preparation of chitinase–chymotrypsin–DMSO buffer (CCD buffer) for enzymatic digestion of larvae

The enzyme from Sigma has been used to assess the effect of limited proteolysis with α-chymotrypsin on the sperm penetration.

생화학적/생리학적 작용

α-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) linked by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. Its pI is 8.75. It selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca²⁺ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α1-antitrypsin, and α2-macroglobulin, 10 mM Cu²⁺ and Hg^2+.

A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met, Leu) on the carboxyl end of the bond.

단위 정의

One unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.

분석 메모

Protein determined by A1%/280

안전성 정보

• 픽토그램: GHS08,GHS07,GHS09
• 신호어: Danger
• 유해 및 위험 성명서: H302,H315,H319,H334,H335,H400
• 예방조치 성명서: P261 - P264 - P273 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• 표적 기관: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• 개인 보호 장비: dust mask type N95 (US), Eyeshields, Faceshields, Gloves