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Hyaluronidase, Streptomyces hyaluronlyticus nov. sp.

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Synonym(s):
Hyaluronidase, Streptomyces hyaluronlyticus nov. sp.
CAS Number:
Enzyme Commission number:
MDL number:

conjugate

conjugate (Glucosaminoglycan)

Quality Level

form

lyophilized powder

specific activity

≥2000 units (A280)

packaging

ampule of ≥100 U (1 U = 1 TRU (Turbidity Reducing Unit))

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

color

white

solubility

0.5 M acetate, pH 6.0: 30 U/mL
water: 30 U/mL

foreign activity

protease ≤0.05 U/ampule (pH 6.0)

shipped in

ambient

storage temp.

2-8°C

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Hyaluronidase from sheep testes Type V, lyophilized powder, ≥1,500 units/mg solid

H6254

Hyaluronidase from sheep testes

Hyaluronidase from bovine testes Type IV-S, powder, suitable for mouse embryo cell culture, 750-3000 units/mg solid

H4272

Hyaluronidase from bovine testes

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

powder

shipped in

ambient

shipped in

-

shipped in

-

shipped in

-

solubility

0.5 M acetate, pH 6.0: 30 U/mL, water: 30 U/mL

solubility

-

solubility

-

solubility

-

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage condition

OK to freeze

storage condition

-

storage condition

-

storage condition

-

General description

Native hyaluronidase from Streptomyces hyaluronlyticus nov. species. Catalyzes the degradation of hyaluronic acid. Produces δ4,5-unsaturated tetra- and hexasaccharides.
Native hyaluronidase from Streptomyces hyaluronlyticus nov. species. Catalyzes the degradation of hyaluronic acid. Produces δ4,5-unsaturated tetra- and hexasaccharides.



Please Note: The appearance of this product can vary from gray cottony fibers to balled up droplets with no change in quality.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One TRU (Turbidity Reducing Unit = 1 U) is defined as the amount of enzyme that will hydrolyze 50% of hyaluronic acid, determined by a 50% reduction in turbidity, in 30 min at 60°C pH 6.0.

Reconstitution

Following reconstitution, store in the refrigerator (4°C). Stock solutions prepared in water are stable for up to 2 weeks at 4°C and stock solutions prepared in 500 mM acetate buffer, pH 6.0, are stable for up to 1 month at 4°C.

Other Notes

Baker, J.R., et al. 2002. Biochem. J.365, 317.
Mogelerskii, M.S. 1964. Fed. Proc. 23, T559.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Jennifer E Rowley et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 68(1), 75-91 (2019-11-13)
Hyaluronan (HA) is a ubiquitous component of the extracellular matrix. The spatial-temporal localization of HA can be visualized in situ using biotinylated HA binding proteins (HABPs). This assay is sensitive to fixation conditions, and there are currently no best practices

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