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APT129

Sigma-Aldrich

Caspase 8 Colorimetric Activity Assay Kit 25, IETD

Caspase-8 Colorimetric Activity Assay Kits provide a simple & convenient means for assaying the activity of caspases that recognize the sequence IETD.

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Synonym(s):
FLICE
eCl@ss:
32161000
NACRES:
NA.84

Quality Level

species reactivity (predicted by homology)

mammals

manufacturer/tradename

Chemicon®

technique(s)

activity assay: suitable

UniProt accession no.

detection method

colorimetric

shipped in

dry ice

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APT171APT173APT131
manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

technique(s)

activity assay: suitable

technique(s)

activity assay: suitable

technique(s)

activity assay: suitable

technique(s)

activity assay: suitable

NCBI accession no.

NM_001080124.1, NM_001080125.1, NM_001228.4, NM_033355.3, NM_033356.3, NM_033358.3

NCBI accession no.

NM_001080124.1, NM_001080125.1, NM_001228.4, NM_033355.3, NM_033356.3, NM_033358.3

NCBI accession no.

NM_001229.2, NM_032996.1

NCBI accession no.

NM_004346.3, NM_032991.2

UniProt accession no.

Q14790

UniProt accession no.

Q14790

UniProt accession no.

P55211

UniProt accession no.

P42574

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

General description

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. Chemicon′s Caspase-8 Colorimetric Activity Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD. The assay is based on spectophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate IETD-pNA. The free pNA can be quantified using a spectrophotometer or a microtiter plate reader at 405 nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-8 activity.

Application

Research Category
Apoptosis & Cancer

Components

5X Cell Lysis Buffer (Part No. 90065): 5 mL

5X Assay Buffer (Part No. 90066): 10 mL

Caspase-8 Substrate (Ac-IETD-pNA) (Part No. 90081): 250 μL of 2.5 mg/mL solution

Caspase-8 Inhibitor (Ac-IETD-CHO) (Part No. 90086): 50 μL of 100 mM (0.05 mg/mL) in DMSO

pNA Standard (Part No. 90085): 250 μL of 10 mM in DMSO

Storage and Stability

Store kit materials at -20°C up to their expiration date.

Special Precautions:

· After thawing reagents, use immediately or aliquot and freeze at -20oC for longer storage. Avoid repeated freeze/thaw cycles.

· The Caspase-8 substrate and pNA standard are especially light sensitive. Maintain these reagents in amber or covered containers.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2


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