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MilliporeSigma

24385C

EX-CELL® CD HEK293 Viral Vector Medium

Chemically defined, animal component-free, dry powder, suitable (for cell culture)

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About This Item

UNSPSC Code:
41116105

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Quality Level

500

form

dry powder

storage condition

protect from light

suitability

suitable (for cell culture)

storage temp.

2-8°C

General description

EX-CELL® CD HEK293 Viral Vector Medium is an animal origin-free, chemically defined cell culture medium for clinical and commercial manufacturing of viral vectors for gene and cell therapies. The medium is intended for processes implementing (polyethylenimine) PEI transfection reagents. EX-CELL CD HEK293 Viral Vector Medium is part of the VirusExpress® Lentiviral Production Platform and the medium of choice for VirusExpress 293T Lentiviral Production Cells (VP001). The medium is also suitable as a standalone solution for other HEK293 and HEK293T cell lines for AAV and lentiviral manufacturing. EX-CELL® CD HEK293 Viral Vector Medium has been formulated to support high suspension cell densities with minimal clumping, high transient transfection efficiency when used with PEI, and high viral titers in shake flask and stirred tank bioreactor formats. Viral vector production using this medium is a scalable, suspension process that does not require a media exchange or feed post-transfection.
EX-CELL® CD HEK293 Viral Vector Medium has been designed with long-term needs for manufacturing in mind, with a sustainable raw material supply chain, custom packaging options, and careful material and manufacturing process selection. The product is produced in an ISO9001-certified facility following the principles of cGMP according to IPEC-PQG.

Packaging

200 L and 50 L pack size in HDPE pail

Preparation Note

EX-CELL CD HEK293 Viral Vector Medium Dry Powder Hydration Instructions: To prepare 1000mL Product: 1. Add 20.41 grams product and 2.0 grams Sodium Bicarbonate to 800mL purified water. 2. Continually stir for 30 minutes. 3. Adjust the pH to a range of 7.0-7.2 using 5N NaOH. Stir for 5 mins. 4. QS to final volume 1000mL with purified water. Use for pH, osmolality, and solubility.

Legal Information

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany
VirusExpress is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation mark
GHS07

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Warning

hcodes

H319

Hazard Classifications

Eye Irrit. 2

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

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Articles

Development and Optimization of a Lentivirus Manufacturing Platform

Learn how development of a suspension adapted HEK293T cell line with accompanying cell culture media can maximize lentivirus production for cell and gene therapies.

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