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I0408

Sigma-Aldrich

Invertase Glycoprotein Standard

BioReagent, from Saccharomyces cerevisiae, for proteomics

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Synonym(s):
Invertase from baker's yeast (S. cerevisiae), β-D-Fructofuranosidase, β-D-Fructofuranoside fructohydrolase, Saccharase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.32

biological source

Saccharomyces cerevisiae

Quality Level

grade

for proteomics

product line

BioReagent

form

lyophilized powder

mol wt

60 kDa

concentration

≥0.5 mg/vial protein (E1%/280)

storage temp.

2-8°C

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This Item
I4504I4753S5391
Sigma-Aldrich

I0408

Invertase Glycoprotein Standard

Invertase from Candida utilis Grade X, ≥300 units/mg solid

I4753

Invertase from Candida utilis

Sucrose ≥99.5% (GC), Grade II, suitable for plant cell culture

S5391

Sucrose

biological source

Saccharomyces cerevisiae

biological source

bakers yeast

biological source

-

biological source

sugar cane

form

lyophilized powder

form

solid

form

solid

form

powder

concentration

≥0.5 mg/vial protein (E1%/280)

concentration

-

concentration

-

concentration

-

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

room temp

mol wt

60 kDa

mol wt

-

mol wt

~260 kDa

mol wt

-

Application

Invertase Glycoprotein Standard has been used:
  • to generate N-linked glycan library
  • as a negative control to study the binding of lectins to high mannose structures
  • for sample pre-treatment in proteomic analyses to study drug-induced toxic epidermal necrolysis
The Invertase Glycoprotein Standard can be used to demonstrate N-glycosylation using PNGase F with both in-solution and in-gel procedures. The extent of deglycosylation can be assessed by mobility shift on SDS-PAGE gels.
Used in the production of confectionary foods and artificial honey.

Biochem/physiol Actions

Invertase hydrolyzes sucrose into glucose and fructose yielding a colorless product, unlike acid hydrolysis which produces colored products.

Other Notes

Invertase is an enzyme that catalyses the hydrolysis of sucrose into fructose and glucose. Invertase Glycoprotein Standard is the periplasmic (glycosylated form, external invertase) with 50% of its mass as polymannan. Since yeast can provide an alternative system for protein glycosylation that is similar to mammalian systems, periplasmic invertase is often used as a model for the study of the function of oligosaccharides in glycoproteins and for studies on glycoprotein biosynthesis.

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

ppe

Eyeshields, Gloves, type N95 (US)


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Acta crystallographica. Section F, Structural biology and crystallization communications, 68(Pt 12), 1538-1541 (2012-11-30)
Saccharomyces cerevisiae invertase (ScInv) is an enzyme encoded by the SUC2 gene that releases β-fructose from the nonreducing termini of various β-D-fructofuranoside substrates. Its ability to produce 6-kestose by transglycosylation makes this enzyme an interesting research target for applications in
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Invertase (E.C.3.2.1.26) from Saccharomyces cerevisiae was covalently immobilized on chitosan nanoparticles prepared by the ionotropic gelation method and activated with glutaraldehyde. The support was characterized and it was studied its load capacity, the influence of the presence of substrate during

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