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MAK152

Sigma-Aldrich

Colorimetric Sphingomyelinase Assay Kit

sufficient for 200 colorimetric tests

Synonym(s):

Neutral Sphingomyelinase Assay Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 200 colorimetric tests

detection method

colorimetric

relevant disease(s)

rheumatological diseases; cancer

Storage temp.

−20°C

Gene Information

human ... SMPD2(6610) , SMPD3(55512) , SMPD4(55627)
mouse ... SMPD2(20598) , SMPD3(58994) , SMPD4(77626)
rat ... SMPD2(83537) , SMPD3(94338) , SMPD4(303790)

General description

Sphingomyelinase (SMase), or sphingomyelin phosphodiesterase, catalyzes the hydrolytic cleavage of sphingolipid to phosphocholine and ceramide, a second messenger involved in multiple cellular responses including the cellular response to stress. There are at least 5 types of SMases identified which are classified according to their cation dependence, cellular location, and pH optima for activity and include the acidic and neutral sphingomyelinase. Neutral SMases, along with the acidic SMases, are thought to be major contributors to the production of ceramide in response to cellular stress.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

This kit is suitable for the detection of sphingomyelinase activity in a variety of samples such as blood and cell extracts or for screening sphingomyelinase inhibitors and activators.

Principle

The Sphingomyelinase Assay Kit provides a simple and direct procedure for measuring neutral SMase activity in a variety of samples such as blood or cell extracts, or for screening SMase activators and inhibitors. SMase activity is determined by a coupled enzyme assay, which results in a colorimetric (655 nm) product proportional to the SMase activity present.

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Danger

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Sens. 1

Storage Class

10 - Combustible liquids

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Certificates of Analysis (COA)

Lot/Batch Number
3511162
3511162
3320752
3320752
318028

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Javad Habibi et al.
Journal of molecular and cellular cardiology, 167, 32-39 (2022-03-26)
Sphingomyelinases ensure ceramide production and play an integral role in cell turnover, inward budding of vesicles and outward release of exosomes. Recent data indicate a unique role for neutral sphingomyelinase (nSMase) in the control of ceramide-dependent exosome release and inflammatory
Wojciech Łuczaj et al.
Antioxidants (Basel, Switzerland), 9(12) (2020-12-02)
UV radiation is a well-established environmental risk factor known to cause oxidative stress and disrupt the metabolism of keratinocyte phospholipids. Cannabidiol (CBD) is a phytocannabinoid with anti-inflammatory and antioxidant effects. In this study, we examined changes in the keratinocyte phospholipid

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