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  • Rationally selected basis proteins: a new approach to selecting proteins for spectroscopic secondary structure analysis.

Rationally selected basis proteins: a new approach to selecting proteins for spectroscopic secondary structure analysis.

Protein science : a publication of the Protein Society (2003-08-22)
Keith A Oberg, Jean-Marie Ruysschaert, Erik Goormaghtigh
ABSTRACT

Protein basis sets have been extensively used as reference data for the determination of protein structure with optical methods such as circular dichroism and infrared spectroscopies. We have taken a new approach to basis protein selection by utilizing three crystal structure classification databases: CATH, SCOP, and PDB_SELECT. Through the use of the information available in these and other online resources, we identified 115 commercially available proteins as potential basis set candidates. By carefully screening the quality of the crystal structures and commercial protein preparations, we obtained a final set of 50 rationally selected proteins (RaSP50) that has been optimized for use in spectroscopic protein structure determination studies. These proteins span the full range of known protein folds as well as alpha-helix and beta-sheet contents, and they represent a more comprehensive variety of fold types than any previous reference set. This report includes a detailed presentation of the reasoning behind the rational protein selection process, a description of the properties of the RaSP50 set, and a discussion of the types of structural and spectral variations that are represented in the set.

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