Skip to Content
MilliporeSigma
  • In type 2 diabetes mellitus glycated albumin alters macrophage gene expression impairing ABCA1-mediated cholesterol efflux.

In type 2 diabetes mellitus glycated albumin alters macrophage gene expression impairing ABCA1-mediated cholesterol efflux.

Journal of cellular physiology (2014-11-22)
Adriana Machado-Lima, Rodrigo T Iborra, Raphael S Pinto, Gabriela Castilho, Camila H Sartori, Erika R Oliveira, Ligia S Okuda, Edna R Nakandakare, Daniel Giannella-Neto, Ubiratan F Machado, Maria Lucia C Corrêa-Giannella, Pietro Traldi, Simona Porcu, Marco Roverso, Annunziata Lapolla, Marisa Passarelli
ABSTRACT

Advanced glycation end products (AGE) are elevated in diabetes mellitus (DM) and predict the development of atherosclerosis. AGE-albumin induces oxidative stress, which is linked to a reduction in ABCA-1 and cholesterol efflux. We characterized the glycation level of human serum albumin (HSA) isolated from poorly controlled DM2 (n = 11) patients compared with that of control (C, n = 12) individuals and determined the mechanism by which DM2-HSA can interfere in macrophage lipid accumulation. The HSA glycation level was analyzed by MALDI/MS. Macrophages were treated for 18 h with C- or DM2-HSA to measure the (14) C-cholesterol efflux, the intracellular lipid accumulation and the cellular ABCA-1 protein content. Agilent arrays (44000 probes) were used to analyze gene expression, and the differentially expressed genes were validated by real-time RT-PCR. An increased mean mass was observed in DM2-HSA compared with C-HSA, reflecting the condensation of at least 5 units of glucose. The cholesterol efflux mediated by apo AI, HDL3 , and HDL2 was impaired in DM2-HSA-treated cells, which was related to greater intracellular lipid accumulation. DM2-HSA decreased Abcg1 mRNA expression by 26%. Abca1 mRNA was unchanged, although the final ABCA-1 protein content decreased. Compared with C-HAS-treated cells, NADPH oxidase 4 mRNA expression increased in cells after DM2-HSA treatment. Stearoyl-Coenzyme A desaturase 1, janus kinase 2, and low density lipoprotein receptor mRNAs were reduced by DM2-HSA. The level of glycation that occurs in vivo in DM2-HSA-treated cells selectively alters macrophage gene expression, impairing cholesterol efflux and eliciting intracellular lipid accumulation, which contribute to atherogenesis, in individuals with DM2.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Trypan Blue, Dye content 60 %, ≥80% (HPLC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 μL
Estimated to ship on March 22, 2025
$196.00
4 x 25 μL
Estimated to ship on March 22, 2025
$404.00
Sigma-Aldrich
Propylene glycol, ≥99.5% (GC), FCC, FG
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 μL
Estimated to ship on March 22, 2025
$196.00
4 x 25 μL
Estimated to ship on March 22, 2025
$404.00
Sigma-Aldrich
Trypan Blue, powder, BioReagent, suitable for cell culture
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 μL
Estimated to ship on March 22, 2025
$196.00
4 x 25 μL
Estimated to ship on March 22, 2025
$404.00
Sigma-Aldrich
Propylene Glycol, meets USP testing specifications
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 μL
Estimated to ship on March 22, 2025
$196.00
4 x 25 μL
Estimated to ship on March 22, 2025
$404.00
Sigma-Aldrich
Acetonitrile solution, contains 10.0% acetone, 0.05% formic acid, 40.0% 2-propanol
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 μL
Estimated to ship on March 22, 2025
$196.00
4 x 25 μL
Estimated to ship on March 22, 2025
$404.00
Supelco
Propylene glycol, Pharmaceutical Secondary Standard; Certified Reference Material
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 μL
Estimated to ship on March 22, 2025
$196.00
4 x 25 μL
Estimated to ship on March 22, 2025
$404.00
Sigma-Aldrich
Acetonitrile solution, contains 0.05 % (w/v) ammonium formate, 5 % (v/v) water, 0.1 % (v/v) formic acid, suitable for HPLC
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 μL
Estimated to ship on March 22, 2025
$196.00
4 x 25 μL
Estimated to ship on March 22, 2025
$404.00
Supelco
Residual Solvent - Acetonitrile, Pharmaceutical Secondary Standard; Certified Reference Material
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 μL
Estimated to ship on March 22, 2025
$196.00
4 x 25 μL
Estimated to ship on March 22, 2025
$404.00
Propylene glycol, European Pharmacopoeia (EP) Reference Standard
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 μL
Estimated to ship on March 22, 2025
$196.00
4 x 25 μL
Estimated to ship on March 22, 2025
$404.00
USP
Propylene glycol, United States Pharmacopeia (USP) Reference Standard
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 μL
Estimated to ship on March 22, 2025
$196.00
4 x 25 μL
Estimated to ship on March 22, 2025
$404.00
Sigma-Aldrich
Acetonitrile solution, contains 0.1 % (v/v) trifluoroacetic acid, suitable for HPLC
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 μL
Estimated to ship on March 22, 2025
$196.00
4 x 25 μL
Estimated to ship on March 22, 2025
$404.00
Sigma-Aldrich
Acetonitrile solution, contains 0.05 % (v/v) trifluoroacetic acid
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 μL
Estimated to ship on March 22, 2025
$196.00
4 x 25 μL
Estimated to ship on March 22, 2025
$404.00
Sigma-Aldrich
Acetonitrile solution, contains 0.1 % (v/v) formic acid, suitable for HPLC
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 μL
Estimated to ship on March 22, 2025
$196.00
4 x 25 μL
Estimated to ship on March 22, 2025
$404.00
Sigma-Aldrich
Trypan Blue solution, 0.4%, liquid, sterile-filtered, suitable for cell culture
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 μL
Estimated to ship on March 22, 2025
$196.00
4 x 25 μL
Estimated to ship on March 22, 2025
$404.00
Supelco
1,2-Propanediol, analytical standard
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 μL
Estimated to ship on March 22, 2025
$196.00
4 x 25 μL
Estimated to ship on March 22, 2025
$404.00
Supelco
Acetonitrile, analytical standard
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 μL
Estimated to ship on March 22, 2025
$196.00
4 x 25 μL
Estimated to ship on March 22, 2025
$404.00
Sigma-Aldrich
Acetonitrile, anhydrous, 99.8%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 μL
Estimated to ship on March 22, 2025
$196.00
4 x 25 μL
Estimated to ship on March 22, 2025
$404.00
Sigma-Aldrich
Acetonitrile, ≥99.5%, ACS reagent
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 μL
Estimated to ship on March 22, 2025
$196.00
4 x 25 μL
Estimated to ship on March 22, 2025
$404.00
Sigma-Aldrich
1,2-Propanediol, puriss. p.a., ACS reagent, ≥99.5% (GC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 μL
Estimated to ship on March 22, 2025
$196.00
4 x 25 μL
Estimated to ship on March 22, 2025
$404.00
Sigma-Aldrich
1,2-Propanediol, ACS reagent, ≥99.5%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 μL
Estimated to ship on March 22, 2025
$196.00
4 x 25 μL
Estimated to ship on March 22, 2025
$404.00
Supelco
Trifluoroacetic acid, analytical standard
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 μL
Estimated to ship on March 22, 2025
$196.00
4 x 25 μL
Estimated to ship on March 22, 2025
$404.00
Sigma-Aldrich
1,2-Propanediol, tested according to Ph. Eur.
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 μL
Estimated to ship on March 22, 2025
$196.00
4 x 25 μL
Estimated to ship on March 22, 2025
$404.00
Sigma-Aldrich
1,2-Propanediol, meets analytical specification of Ph. Eur., BP, USP, ≥99.5%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 μL
Estimated to ship on March 22, 2025
$196.00
4 x 25 μL
Estimated to ship on March 22, 2025
$404.00
Sigma-Aldrich
Trypan Blue solution, 0.4%, for microscopy
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 μL
Estimated to ship on March 22, 2025
$196.00
4 x 25 μL
Estimated to ship on March 22, 2025
$404.00
Sigma-Aldrich
Acetonitrile, biotech. grade, ≥99.93%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 μL
Estimated to ship on March 22, 2025
$196.00
4 x 25 μL
Estimated to ship on March 22, 2025
$404.00
Sigma-Aldrich
Acetonitrile, ACS reagent, ≥99.5%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 μL
Estimated to ship on March 22, 2025
$196.00
4 x 25 μL
Estimated to ship on March 22, 2025
$404.00
Sigma-Aldrich
Acetonitrile, suitable for DNA synthesis, ≥99.9% (GC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 μL
Estimated to ship on March 22, 2025
$196.00
4 x 25 μL
Estimated to ship on March 22, 2025
$404.00
Sigma-Aldrich
Acetonitrile, HPLC Plus, ≥99.9%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 μL
Estimated to ship on March 22, 2025
$196.00
4 x 25 μL
Estimated to ship on March 22, 2025
$404.00
Sigma-Aldrich
Acetonitrile, suitable for HPLC, gradient grade, ≥99.9%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 μL
Estimated to ship on March 22, 2025
$196.00
4 x 25 μL
Estimated to ship on March 22, 2025
$404.00
Sigma-Aldrich
Acetonitrile, ReagentPlus®, 99%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 μL
Estimated to ship on March 22, 2025
$196.00
4 x 25 μL
Estimated to ship on March 22, 2025
$404.00