Skip to Content
MilliporeSigma
  • In vivo and ex vivo proofs of concept that cetuximab conjugated vitamin E TPGS micelles increases efficacy of delivered docetaxel against triple negative breast cancer.

In vivo and ex vivo proofs of concept that cetuximab conjugated vitamin E TPGS micelles increases efficacy of delivered docetaxel against triple negative breast cancer.

Biomaterials (2015-06-18)
Rajaletchumy Veloo Kutty, Sing Ling Chia, Magdiel I Setyawati, Madaswamy S Muthu, Si-Shen Feng, David Tai Leong
ABSTRACT

In this study we examined the efficacy of our micellar system in xenograft models of triple negative breast cancers and explored the effect of the micelles on post-treatment tumours in order to elucidate the mechanism underlying the nanomedicine treatment in oncology. Here, we developed docetaxel-loaded vitamin E D-α-tocopheryl polyethylene glycol succinate (TPGS) micelles, of which the surface modified with cetuximab ligands for targeting epidermal growth factor receptors (EGFR) that are overexpressed in MDA-MB-231 breast cancer cells. The targeting micelles accumulated in the tumours immediately after the intravenous injection and retained for at least 24 h. The successful delivery of docetaxel into the tumours by the targeting micelles was shown by the greater degree of tumour growth inhibition than that for Taxotere(®) after the 15-day treatment. Furthermore, the explanted tumour culture study involving gene analysis and immunohistochemistry staining indicated that the in vivo micelle treatment induced cell cycle arrest and attenuated cell proliferation. In addition, the targeting and non-targeting micellar formulations brought about anti-angiogenesis and anti-migration effects. Overall, both the in vivo and ex vivo data increased the confidence that our micellar formulations effectively targeted and inhibited EGFR-overexpressing MDA-MB-231 tumours.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Eosin Y, 75% (HPLC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 ea
In Stock
Details...
$84.80
Sigma-Aldrich
Eosin Y solution, 5 wt. % in H2O
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 ea
In Stock
Details...
$84.80
Sigma-Aldrich
Eosin Y, SAJ special grade, ≥85.0%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 ea
In Stock
Details...
$84.80
Sigma-Aldrich
Hydrogen peroxide solution, SAJ first grade, ≥30.0%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 ea
In Stock
Details...
$84.80
Sigma-Aldrich
Eosin Y, Dye content ~99 %
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 ea
In Stock
Details...
$84.80
Sigma-Aldrich
(±)-α-Tocopherol, synthetic, ≥96% (HPLC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 ea
In Stock
Details...
$84.80
Sigma-Aldrich
D-Luciferin, synthetic, BioXtra, ≥99% (HPLC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 ea
In Stock
Details...
$84.80
Sigma-Aldrich
D-Luciferin, synthetic
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 ea
In Stock
Details...
$84.80
Sigma-Aldrich
Eosin Y disodium salt, Dye content ≥85 %
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 ea
In Stock
Details...
$84.80
Sigma-Aldrich
Eosin Y disodium salt, certified by the Biological Stain Commission
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 ea
In Stock
Details...
$84.80
Sigma-Aldrich
Hydrogen peroxide solution, 30 % (w/w) in H2O, contains stabilizer
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 ea
In Stock
Details...
$84.80
Sigma-Aldrich
Propidium iodide, ≥94.0% (HPLC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 ea
In Stock
Details...
$84.80
Sigma-Aldrich
Hydrogen peroxide solution, contains potassium stannate as inhibitor, 30-32 wt. % in water, semiconductor grade, 99.999% trace metals basis
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 ea
In Stock
Details...
$84.80
Sigma-Aldrich
Hydrogen peroxide solution, 34.5-36.5%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 ea
In Stock
Details...
$84.80
Sigma-Aldrich
Ethanol Fixative 80% v/v, suitable for fixing solution (blood films)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 ea
In Stock
Details...
$84.80
Sigma-Aldrich
Ethanol, JIS first grade, 94.8-95.8%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 ea
In Stock
Details...
$84.80
Sigma-Aldrich
Ethanol, JIS special grade, 94.8-95.8%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 ea
In Stock
Details...
$84.80
Sigma-Aldrich
3,3′-Diaminobenzidine, 97% (HPLC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 ea
In Stock
Details...
$84.80
Sigma-Aldrich
L-Lysine monohydrochloride, reagent grade, ≥98% (HPLC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 ea
In Stock
Details...
$84.80
Sigma-Aldrich
Hematoxylin, certified by the Biological Stain Commission
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 ea
In Stock
Details...
$84.80
Sigma-Aldrich
(+)-α-Tocopherol, Type VI, from vegetable oil, liquid (≥0.88M based on potency, density and molecular wt.), BioReagent, suitable for insect cell culture, ≥1000 IU/g
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 ea
In Stock
Details...
$84.80
Sigma-Aldrich
Hematoxylin
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 ea
In Stock
Details...
$84.80
Sigma-Aldrich
(+)-α-Tocopherol, from vegetable oil, Type V, ~1000 IU/g
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 ea
In Stock
Details...
$84.80
Sigma-Aldrich
Propidium iodide solution
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 ea
In Stock
Details...
$84.80
Sigma-Aldrich
L-Lysine monohydrochloride, from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, 98.5-101.0%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 ea
In Stock
Details...
$84.80
Sigma-Aldrich
L-Lysine monohydrochloride, BioUltra, ≥99.5% (AT)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 ea
In Stock
Details...
$84.80
Sigma-Aldrich
1,2-Dichloroethane, SAJ first grade, ≥99.0%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 ea
In Stock
Details...
$84.80
Sigma-Aldrich
1,2-Dichloroethane, JIS special grade, ≥99.5%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 ea
In Stock
Details...
$84.80
Sigma-Aldrich
α-Tocopherol, ≥95.5%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 ea
In Stock
Details...
$84.80
Sigma-Aldrich
N-Hydroxysuccinimide, 98%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 ea
In Stock
Details...
$84.80