04-148

Anti-CLEC9A Antibody, clone 10B4

clone 10B4, from rat

• 別名: C-type lectin domain family 9 member A, Dendritic cell natural killer lectin group receptor 1, CLEC9A, CLEC
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• クローン: 10B4, monoclonal
• application: FACS; WB
• 化学種の反応性: mouse
• テクニック: flow cytometry: suitable; western blot: suitable
• citations: 1

特性

• 由来生物: rat
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 10B4, monoclonal
• 化学種の反応性: mouse
• テクニック: flow cytometry: suitable; western blot: suitable
• アイソタイプ: IgG2aκ
• NCBIアクセッション番号: NP_001192292
• UniProtアクセッション番号: Q8BRU4
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: mouse ... Clec9A(232414)

詳細

詳細

CLEC9A, also known as Dendritic cell natural killer lectin group receptor 1 (DNGR-1), and encoded by the gene Clec9a/Dngr-1, functions as an endocytic receptor on a small subset of myeloid cells specialized for the uptake and processing of material from dead cells. CLEC9A functions as a SYK-coupled C-type lectin receptor to mediate sensing of necrosis by the principal dendritic-cell subset involved in regulating cross-priming to cell-associated antigens. CLEC9A recognizes filamentous form of actin in association with particular actin-binding domains of cytoskeletal proteins, including spectrin, exposed when cell membranes are damaged, and mediates the cross-presentation of dead-cell associated antigens in a Syk-dependent manner. CLEC9A is localized to the plasma membrane. CLEC9A is expressed in dendritic cells and has multiple isoforms; isoform 4 is expressed at high levels by CD8+ dendritic cells and at low levels by plasmacytoid DCs but not by other hematopoietic cells.

免疫原

KLH-conjugated linear peptide corresponding to mouse CLEC9A.

アプリケーション

Research Category
炎症及び免疫

Research Sub Category
免疫グロブリン及び免疫

Detect CLEC9A using this rat monoclonal antibody, Anti-CLEC9A Antibody, clone 10B4 validated for use in western blotting & Flow Cytometry.

Flow Cytometry Analysis: A representative lot from an independent laboratory detected CLEC9A in mouse Conventional Dendritic cells and on most Plasmacytoid Dendritic cells (Caminschi, I., et al. (2008). Blood. 112(8):3264-3273.).

品質

Evaluated by Western Blotting in mouse lymph node tissue lysate.

Western Blotting Analysis: 1 μg/mL of this antibody detected CLEC9A in 10 μg of mouse lymph node tissue lysate.

ターゲットの説明

~27 kDa and ~16 kDa observed. Uniprot describes five isoforms at 27 kDa, 27 kDa, 30 kDa, 20 kDa, and 17 kDa

物理的形状

Protein G Purified

Format: Purified

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable