537059

Propidium Iodide

Membrane impermeable DNA intercalator.

• 別名: Propidium Iodide
• 実験式（ヒル表記法）: C₂₇H₃₄I₂N₄
• CAS番号: 25535-16-4
• 分子量: 668.39
• MDL番号: MFCD00011921
• UNSPSCコード: 12352200
• NACRES: NA.84

特性

• 品質水準: 100
• 形状: solid
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; protect from light
• 色: dark red
• 入力: sample type intact cells
• 蛍光検出: λ_ex ~536 nm; λ_em ~617 nm
• 輸送温度: ambient
• 保管温度: 2-8°C
• InChI: 1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1
• InChI Key: XJMOSONTPMZWPB-UHFFFAOYSA-M

詳細

詳細

Extinction coefficient(493 nm): 5900 M ^-1cm^-1;(287 nm): 55,700 M ^-1cm^-1;

Membrane impermeable DNA intercalator. Has red fluorescence at 488 nm. Useful for flow cytometry for staining apoptotic cells and nuclei. Can be used to differentiate between apoptotic and necrotic cell death while stains only necrotic cells. Can be used together with common green fluorescent probes labeled with fluorescein isothiocyanate (FITC).

Membrane impermeable DNA intercalator. Has red fluorescence at 488 nm. Useful for staining apoptotic cells and nuclei by flow cytometry. Can be used to differentiate between apoptotic and necrotic cell death. Can be used together with common green fluorescent probes labeled with fluorescein isothiocyanate. Has the following spectral properties: extinction coefficient: 5,900 M^-1cm^-1 (493 nm); 55,700 M^-1cm^-1 (287 nm); 13,800 M^-1cm^-1 (252.5 nm); excitation maximum: 536 nm; emission maximum: 617 nm.

生物化学的／生理学的作用

Cell permeable: no

Primary Target
Membrane impermeable DNA intercalator

Product does not compete with ATP.

Reversible: no

警告

Toxicity: Irritant (B)

調製ノート

Solubility may be improved by first dissolving in a very small amount of DMSO, then adding material to an aqueous solution.

再構成

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

その他情報

Belloc, F., et al. 1994. Cytometry 17, 59.
Crompton, T., et al. 1992. Biochem. Biophys. Res. Commun. 183, 532.
Darzynkiewicz, Z., et al. 1992. Cytometry13, 795.
de Caestecker, M.P., et al. 1992. J. Immunol. Methods154, 11.
Pollice, A.A., et al. 1992. Cytometry13, 432.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• ピクトグラム: GHS08
• シグナルワード: Warning
• 危険有害性情報: H341
• 注意書き: P201 - P202 - P280 - P308 + P313 - P405 - P501
• 危険有害性の分類: Muta. 2
• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable