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形状
solid
品質水準
使用法
sufficient for ~50 tests
分子量
105.99 g/mol
メーカー／製品名
Roche
テクニック
activity assay: suitable
色
white to light yellow-brown
溶解性
water: soluble ~210 g/L at 68 °F (Stop reagent)
soluble ~71 g/L at 32 °F (stop reagent)
water: soluble (Buffer substance, lyophilized substrate)
適合性
suitable for enzyme test
アプリケーション
life science and biopharma
保管温度
room temp
詳細
N-acetyl-β-D-glucosaminidase (NAG) is mainly used in the colorimetric assay for the determination of N-acetyl-β-D-glucosaminidase in urine. NAG hydrolyzes 3-cresolsulfonphthaleinyl-N-acetyl-D-glucosaminide to release 3-cresolsulfonphthalein, which is measured photometrically at 580nm. It is an intracellular lysosomal enzyme, present in the S3 segment of proximal tubular cells and distal nephron. Presence of NAG in the urine shows an organelle impairment in proximal tubule. Level of NAG rises in individuals over 70 years of age.
アプリケーション
N-acetyl-β-D-glucosaminidase (NAG) has been used for preparing standards for the determination of N-acetyl-β-D-glucosaminidase in urine in life-science research applications.
包装
1 kit containing 3 components
調製ノート
Working solution: Preparation of solutions:
Storage conditions (working solution): Stability of solutions:
Solution II is stable for 1 month when stored at 2 to 8 °C, protected from light.
Solution III is stable for 1 month stored at 2 to 8 °C.
Stability of the sample:
The activity determination of the N-acetyl-β-D-glucosaminidase (NAG) should be carried out directly after collecting the sample. Turbid urines should be centrifuged and the supernatant decanted. NAG is stable for one week at 2 to 8 °C and for one month when stored at -15 to -25 °C.
- Buffer solution
Dissolve the contents of bottle 1 with 55 ml double-distilledwater. - Substrate solution
Dissolve the contents of bottle 2 with 55 ml solution I. - Stop reagent
Dissolve the contents of bottle 3 with 110 ml double-distilled water.
Storage conditions (working solution): Stability of solutions:
Solution II is stable for 1 month when stored at 2 to 8 °C, protected from light.
Solution III is stable for 1 month stored at 2 to 8 °C.
Stability of the sample:
The activity determination of the N-acetyl-β-D-glucosaminidase (NAG) should be carried out directly after collecting the sample. Turbid urines should be centrifuged and the supernatant decanted. NAG is stable for one week at 2 to 8 °C and for one month when stored at -15 to -25 °C.
保管および安定性
Keep container tightly closed in a dry and well-ventilated place.
その他情報
For life science research only. Not for use in diagnostic procedures.
キットの構成要素のみ
製品番号
詳細
- Buffer Substance consisting of citric acid and potassium citrate
- Lyophilized Substrate consisting of sodium 3-cresolsulfonphthaleinyl-N-acetyl-β-D-glucosaminide and borax
- Stop Reagent consisting of sodium carbonate
シグナルワード
Danger
危険有害性情報
危険有害性の分類
Eye Irrit. 2 - Repr. 1B - STOT SE 3
ターゲットの組織
Respiratory system
保管分類コード
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 2
引火点(°F)
does not flash
引火点(℃)
does not flash
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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