94310

ウリカーゼ from Bacillus fastidiosus

lyophilized, ~9 U/mg

• 別名: Urate oxidase
• CAS番号: 9002-12-4
• Enzyme Commission number: 1.7.3.3 (BRENDA, IUBMB)
• MDL番号: MFCD00082126
• UNSPSCコード: 12352204
• PubChem Substance ID: 57653596
• NACRES: NA.54

特性

• 由来生物: Bacillus sp. (B. fastidiosus)
• 品質水準: 300
• 形状: powder
• 品質: lyophilized
• 比活性: ~9 U/mg
• 保管条件: dry at room temperature
• 濃度: 90—100%
• 色: white to faint beige
• 溶解性: 5 mg/mL, clear, colorless to very faintly yellow (in enzyme dilution buffer)
• アプリケーション: detection
• 保管温度: −20°C
• SMILES記法: N.OCC1OC(C(O)C1OP(O)(=O)OCC2OC(C(O)C2O)N3C=CC(=O)NC3=O)N4C=CC(=O)NC4=O
• InChI: 1S/C18H23N4O14P.H3N/c23-5-7-14(13(28)16(34-7)22-4-2-10(25)20-18(22)30)36-37(31,32)33-6-8-11(26)12(27)15(35-8)21-3-1-9(24)19-17(21)29;/h1-4,7-8,11-16,23,26-28H,5-6H2,(H,31,32)(H,19,24,29)(H,20,25,30);1H3
• InChI Key: VPXDHZMLJOJKOX-UHFFFAOYSA-N

詳細

詳細

Research area: Cell signaling

Uricase is present in many organisms, such as, archaea, bacteria, and eukaryotes. The Gut microbiome is the major source of uricase, a homotetrameric enzyme containing four identical 34 kDa subunits.

アプリケーション

Uricase may be used for the determination of uric acid in biological fluids. It may be helpful in the spectrophotometric determination of purines and purine nucleosides.

生物化学的／生理学的作用

Uricase catalyzes the oxidation of uric acid to 5-hydroxyisourate, which subsequently forms (S)-allantoin. It is involved in purine metabolism, urate degradation and (S)-allantoin formation The absence of functional uricase in humans has significant physiological and pathophysiological implications. It has been associated with higher serum uric acid levels, leading to an increased risk of conditions related to uric acid crystallization, such as gout. Uricase has emerged as an important therapeutic target for the treatment of conditions related to hyperuricemia, such as gout.

単位の定義

1 unitは、pH 9.1、37°Cで、1.0 μmoleの尿酸を酸化する酵素量に相当します。

その他情報

体液中の尿酸測定や、プリン、プリンヌクレオシドの分光光度測定については参考文献をご覧ください。

安全性情報

• ピクトグラム: GHS08
• シグナルワード: Danger
• 危険有害性情報: H319,H334,H360FD
• 注意書き: P202 - P261 - P264 - P280 - P305 + P351 + P338 - P308 + P313
• 危険有害性の分類: Eye Irrit. 2 - Repr. 1B - Resp. Sens. 1
• 保管分類コード: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, type N95 (US)