F3133

繊維芽細胞増殖因子 ウシ下垂体由来

lyophilized, suitable for cell culture

• 別名: FGF
• CAS番号: 62031-54-3
• MDL番号: MFCD00166724
• UNSPSCコード: 12352202
• NACRES: NA.77

特性

• product name: 繊維芽細胞増殖因子 ウシ下垂体由来, suitable for cell culture
• 由来生物: bovine pituitary glands
• 品質水準: 200
• 形状: lyophilized powder
• 有効性: 1-100 ng/mL
• 分子量: 16.4 kDa
• 包装: pkg of 10 and 5X10 μg
• 保管条件: avoid repeated freeze/thaw cycles
• テクニック: cell culture | mammalian: suitable
• 不純物: endotoxin, tested
• 色: white to off-white
• 保管温度: −20°C

詳細

詳細

Fibroblast growth factors (FGF) are glycoproteins concealed in the extracellular matrix and cell surface. There are around 22 different types of FGFs known. These factors weigh in the range of 17 to 34kDa and they possess a conserved sequence of 120 amino acids.

アプリケーション

A natural mitogenic growth factor used in cell culture applications to study cell signaling and to promote fibroblast and endothelial cell growth and survival.

Fibroblast Growth Factor from bovine pituitary has been used in in vitro testicular cell culture. It has also been used as a component of myogenic culture medium.

生物化学的／生理学的作用

Fibroblast growth factors (FGF) actively participate in cellular responses including embryogenesis and organogenesis. It specifically controls cell proliferation, differentiation and migration. FGF is known to regulate nervous system functions, tissue repair mechanism and wound healing. It also controls angiogenesis of tumor cells. Abnormal FGF mediated signaling induces tumor angiogenesis, by stimulating cancer cell proliferation and survival. FGFs carries out its function by mediating through four receptor tyrosine kinases : FGF-receptors FGFR1, FGFR2, FGFR3 and FGFR4.

品質

本製品の主なマイトジェン活性成分はFGF2ですが、タンパク質単位重量あたりの活性は精製FGF2より低くなっています。

物理的形状

凍結乾燥品(0.2 μmフィルタ-で濾過したPBS, 0.1 mg/mL BSA含有)

調製ノート

Gospodarowiczの方法を改変して腺を精製しました。

アナリシスノート

生物活性は3日間のMTT分析で測定されます。

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, type N95 (US)