NUC201

Nuclei Isolation Kit: Nuclei PURE Prep

sufficient for 15 nuclei preparations (~1-10×10⁷ cells or 1g of tissue per preparation)

• 동의어(들): Sucrose centrifugation nuclei isolation
• UNSPSC 코드: 12352207
• NACRES: NA.32

속성

• 사용: sufficient for 15 nuclei preparations (~1-10×10⁷ cells or 1g of tissue per preparation)
• Quality Level: 200
• 포장: pkg of 1 kit
• 저장 조건: dry at room temperature
• 응용 분야: cell analysis
• 외래 활성: nuclease and protease, free
• 배송 상태: wet ice
• 저장 온도: 2-8°C

설명

애플리케이션

For preparation of pure nuclei and fragile nuclei from cell lines and solid tissues.

생화학적/생리학적 작용

The protocol incorporates centrifugation through a dense sucrose cushion to protect nuclei and strip away cytoplasmic contaminants. High yield has been obtained from common cell lines (Jurkat, HFN7.1, COS7, HEK293 and MDCK) and tissues (spleen and liver). These preparations are suitable for many cell biology applications, e.g., as a source of nuclear components such as chromatin, genomic DNA, histones, and nuclear RNA/RNP, produces nuclei for in vitro apoptosis assays, and functional studies such as examination of the transcriptional status of cells.

The protocol incorporates centrifugation through a dense sucrose cushion to protect nuclei and strip away cytoplasmic contaminants. The sucrose concentration that is suitable for a particular cell type is determined empirically by the user. The sucrose concentrate and sucrose cushion buffer give the user flexibility to modify the density of the sucrose cushion as appropriate. High yield has been obtained from common cell lines (Jurkat, HFN7.1, COS7, HEK293 and MDCK) and tissues (spleen and liver). These preparations are suitable for many cell biology applications, e.g., as a source of nuclear components such as chromatin, genomic DNA, histones, and nuclear RNA/RNP, produces nuclei for in vitro apoptosis assays, and functional studies such as examination of the transcriptional status of cells.

키트 구성품 전용

• Nuclei PURE Lysis Buffer 180 mL

안전성 정보

• 픽토그램: GHS05,GHS09
• 신호어: Danger
• 유해 및 위험 성명서: H315,H318,H410
• 예방조치 성명서: P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313
• Hazard Classifications: Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2
• Storage Class Code: 10 - Combustible liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable