GC Analysis of Organophosphorus Pesticides (OP-Pest) in Cabbage, Onion, and Mushrooms on SLB-5ms after SPE using Supelcleanโข ENVI-Carbโข II/PSA
Materials
SPE ํ๋ธ ๋๋ ํ๋ ์ดํธ
Supelcleanโข ENVI-Carbโข II/PSA SPE Tube
bed B 500 mg (Supelcleanโข PSA SPE), bed A 500 mg (Supecleanโข ENVI-Carb-II SPE), volume 6 mL, pk of 30๋ถ์ ์ปฌ๋ผ
CONDITIONS
sample preparation
SPE (Solid Phase Extraction)
sample/matrix
10 g homogenized food sample (green onions, apples, Napa cabbage, button mushrooms)
SPE tube/cartridge
Supelclean ENVI-Carb II/PSA SPE Tube (54067-U)
condition
5 mL acetone:toluene (65:35)
sample addition
1 mL sample extract
elution
10 mL of acetone:toluene (65:35)
eluate post-treatment
Evaporate fraction to 0.5 mL then adjust volume to 1 mL with ethyl acetate for GC-NPD analysis
column
SLB-5ms, 30 m x 0.25 mm I.D., 0.25 μm (28471-U)
oven
110 ยฐC (2 min), 2.5 ยฐC/min to 205 ยฐC, 10 ยฐC/min to 310 ยฐC (10 min)
inj. temp.
250 ยฐC
detector
NPD, 310 ยฐC
carrier gas
helium, 0.9 mL/min constant flow
injection
2 μL, splitless
liner
4 mm I.D. dual tapered
์ค๋ช
์ ์กฐ ๋ฉ๋ชจ
Sample pretreatment: To a 10 g sample of homogenized food (green onions, apples, Napa cabbage, button mushrooms) was added 10 mL acetonitrile and 4 g magnesium sulfate (that had been heated at 550 ยฐC) and 1 g sodium chloride. After centrifugation, the supernatant was removed and mixed with 1 g magnesium sulfate and centrifuged. 5 mL of the supernatant was removed and evaporated to 1 mL for SPE loading.
๋ถ์ ๋ฉ๋ชจ
Organophosphorous pesticide use in agriculture is widespread due to the fact that they are more amenable to environmental degradation in comparison to organochlorine or organonitrogen compounds. There are a large number of pesticides in the organophosphorous group, and because of their potential health effects, they are of particular concern on produce imported from areas in which they are commonly used. This chromatogram illustrates the separation of 63 of these pesticides by GC on the SLB-5ms using nitrogen phosphorus detection (NPD).
๋ฒ์ ์ ๋ณด
ENVI-Carb is a trademark of Sigma-Aldrich Co. LLC
Supelclean is a trademark of Sigma-Aldrich Co. LLC