GC Analysis of Trichloroanisole (TCA) and Precursors on SLBยฎ-5ms after SPME using 100 ฮผm a PDMS Fiber
Materials
SPME ํ์ด๋ฒ
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CONDITIONS
sample/matrix
each analyte at 100 ng/L in a 12% ethanol solution
SPME fiber
metal fiber coated with 100 μm PDMS (57928-U)
extraction
headspace, 50 ยฐC for 30 min, with stirring
desorption process
250 ยฐC for 3 min
column
SLB-5ms, 30 m x 0.25 mm I.D., 0.25 μm (28471-U)
oven
50 ยฐC (1 min), 25 ยฐC/min to 280 ยฐC
inj. temp.
250 ยฐC
detector
GC/MS, ECD, 290 ยฐC
carrier gas
helium, 1.5 mL/min, constant
injection
splitless/split, closed 2 min
liner
0.75 mm I.D. SPME
sample
Wine cork
์ค๋ช
๋ถ์ ๋ฉ๋ชจ
2,4,6-Trichloroanisole (TCA) is a phenolic compound that, if present, gives wine an unpleasant odor often described as musty, woody, dank, and acrid. Human sensory thresholds for TCA are very low, estimated to be approximately 5 parts per trillion. Natural phenolic compounds in cork can convert into TCA when the cork is exposed to chlorine-containing bleach. TCA contamination can also derive from fungi growing on the grapes during fermentation. In this application, a 100 μm PDMS fiber was used to extract TCA from the headspace above a spiked wine sample prior to analysis by GC. The SLB-5ms GC columns provide consistently low-bleed, inert, efficient, and durable separations.
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SLB is a registered trademark of Merck KGaA, Darmstadt, Germany