HPLC Analysis of Immunoglobulin G (IgG) on Zenix^® SEC-150 versus Zenix^® SEC-300, Effect of Pore Size on Resolution

column

Zenix SEC-150, 30 cm x 4.6 mm I.D., 3 μm, 150 Å particles (Z777016)

column

Zenix SEC-300, 30 cm x 4.6 mm I.D., 3 μm, 300 Å particles (Z777028)

mobile phase

150 mM sodium phosphate, pH 7.0

flow rate

1 mL/min

detector

UV, 214 nm

injection

10 μL

sample

1 mg/mL, IgG