93350

Trizma^® base

99.7% (T), puriss. p.a.

• Synonym(s): 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol
• สูตรเชิงเส้น: NH₂C(CH₂OH)₃
• CASE番号: 77-86-1
• น้ำหนักโมเลกุล: 121.14
• UNSPSC Code: 12352104
• NACRES: NA.25
• PubChem Substance ID: 57653537
• EC Number: 201-064-4
• Beilstein/REAXYS Number: 741883
• MDL number: MFCD00004679

คุณสมบัติ

• description: aminopeptidase substrate
• Quality Level: 200
• grade: puriss. p.a.
• assay: 99.7% (T)
• form: crystalline
• ign. residue: ≤0.01% (as SO₄)
• pH: 10.5-11.5 (25 °C, 10% in H₂O)
• useful pH range: 7-9
• pKa (25 °C): 8.1
• bp: 219-220 °C/10 mmHg (lit.)
• mp: 167-172 °C (lit.), 168-172 °C
• solubility: water: soluble (678 g/l at 20 °C)
• anion traces: chloride (Cl^-): ≤5 mg/kg, sulfate (SO₄^2-): ≤5 mg/kg
• cation traces: Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Na: ≤50 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Zn: ≤5 mg/kg
• λ: 10 % in H₂O
• UV absorption: λ: 230 nm A_max: ≤0.6, λ: 260 nm A_max: ≤0.05, λ: 280 nm A_max: ≤0.04, λ: 430 nm A_max: ≤0.01
• SMILES string: NC(CO)(CO)CO
• InChI: 1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
• InChI key: LENZDBCJOHFCAS-UHFFFAOYSA-N

คำอธิบาย

General description

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

Application

Tris base has been used:

• In the preparation of TAE buffer for TAE agarose gel used to run PCR products by gel electrophoresis
• As a component of sample buffer for SDS-PAGE
• In the preparation of IEC (ion-exchange chromatography) buffer

Other Notes

Easily compare specifications for Trizma products with the Trizma specification table.

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

ข้อมูลความปลอดภัย

• คลาสการจัดเก็บ: 11 - Combustible Solids
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves