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A-004-M

Sigma-Aldrich

Poly-L-Ornithine Solution (0.01%)

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Synonym(s):
Poly-L-Ornithine Solution (0.01%), A-004-M, Sigma-Aldrich
eCl@ss:
32160801
NACRES:
NA.75

needle size

2.0 mm

Quality Level

sterility

sterile

form

liquid

mol wt

Mw 78000 Da

manufacturer/tradename

NovaSeptum®

concentration

0.01 % (w/v)
0.1 mg/mL

technique(s)

cell attachment: suitable
cell culture | mammalian: suitable

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This Item
A-005-MO1136P4957
form

liquid

form

liquid

form

-

form

solution

mol wt

Mw 78000 Da

mol wt

Mw 84000 Da

mol wt

antigen 53 kDa

mol wt

30,000-70,000

concentration

0.01 % (w/v), 0.1 mg/mL

concentration

0.01 % (w/v), 0.1 mg/mL

concentration

-

concentration

0.01%

technique(s)

cell attachment: suitable, cell culture | mammalian: suitable

technique(s)

cell attachment: suitable, cell culture | mammalian: suitable

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, microarray: suitable, western blot: 1:100

technique(s)

cell culture | mammalian: suitable

Quality Level

100

Quality Level

100

Quality Level

200

Quality Level

200

General description

Research Category: Stem Cell Research

Research Sub Category: Adhesion (CAMs)

Application

Poly-L-Ornithine Solution (0.01%) has been used to coat:
  • glass-bottom plates for cell plating in a research study investigating the localization of cMET in MDA-MB-468 cells using confocal microscopy
  • culture dishes for adult human neural stem cells (ahNSCs) primary culture
  • 6-well plates for induced pluripotent stem cells (iPSCs) culture and neuronal differentiation

Quality

Solution is clear with no particulates present. Solution is sterile and suitable for cell culture applications.

Physical form

Product is presented in sterile water at 0.01% w/v (0.1mg/ml). Product is filtered through a 0.2 micron filter before freezing.

Preparation Note

1. Thaw Poly-L-Ornithine solution at room temperature.

2. Dilute Poly-L-Ornithine solution to the desired concentration in sterile water.

3. Fully coat the cell culture surface with diluted Poly-L-Ornithine solution. Use 5 mL volume for 6-cm plates and 10 mL volume for 10-cm plates and T75 flasks.

4. Allow the cell culture vessel to sit at room temperature overnight.

5. Aspirate the Poly-L-Ornithine solution the following day and rinse the vessel with sterile water followed by coating with desired ECM protein. Optimal coating concentrations must be determined by the end user. Typical coating concentrations range from 10ug/ml to 100ug/ml based on cell type and application.

Storage and Stability

Store at -20°C for up to 4 months from date of receipt. Avoid multiple freeze thaw cycles to maintain product integrity.

Legal Information

NOVASEPTUM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Discovery of novel cMET-targeting antibody Fab drug conjugates as potential treatment for solid tumors with highly expressed cMET
Expert opinion on biological therapy, 1137-1149 (2023)
Ryan S Hippman et al.
ACS chemical neuroscience, 14(24), 4363-4382 (2023-12-10)
Autophagy is a major catabolic degradation and recycling process that maintains homeostasis in cells and is especially important in postmitotic neurons. We implemented a high-content phenotypic assay to discover small molecules that promote autophagic flux and completed target identification and
Geun-Hyoung Ha et al.
Aging, 14(22), 8944-8969 (2022-11-30)
Mismatches between pre-clinical and clinical results of stem cell therapeutics for ischemic stroke limit their clinical applicability. To overcome these discrepancies, precise planning of pre-clinical experiments that can be translated to clinical trials and the scientific elucidation of treatment mechanisms

Articles

Discover L-ornithine, an important amino acid supplement, explore how L-ornithine is used in CHO and iPSC culture, and discover how poly-L-ornithine is used to enhance cell attachment.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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